Revisiting higher-order and large-scale chromatin organization
Revisiting higher-order and large-scale chromatin organization
复制标题
DOI：
10.1016/j.ceb.2012.03.003
复制
发表时间：
2012-06-01
影响因子：
7.5
通讯作者：
Belmont, Andrew S.
中科院分区：
文献类型：
--
作者：
Bian, Qian;Belmont, Andrew S.
在过去的几年里，人们越来越重视高水平染色质折叠的可塑性。四个不同的'30 nm'染色质纤维结构已被确定，而新的原位成像方法质疑的普遍性，30 nm染色质纤维作为构建块染色体折叠在体内。基于3C的方法提供了一种非微观的基因组方法来研究染色体折叠，同时揭示了传统的分层染色质折叠模型难以适应的大量长距离顺式相互作用。最近基于显微镜的研究表明，复杂的拓扑结构共存于线性间期染色体结构。这些结果要求重新评估传统的模型，更高层次的染色质折叠。
The past several years has seen increasing appreciation for plasticity of higher-level chromatin folding. Four distinct '30 nm' chromatin fiber structures have been identified, while new in situ imaging approaches have questioned the universality of 30 nm chromatin fibers as building blocks for chromosome folding in vivo. 3C-based approaches have provided a non-microscopic, genomic approach to investigating chromosome folding while uncovering a plethora of long-distance cis interactions difficult to accommodate in traditional hierarchical chromatin folding models. Recent microscopy based studies have suggested complex topologies co-existing within linear interphase chromosome structures. These results call for a reappraisal of traditional models of higher-level chromatin folding.