Tumor Suppressor RARRES1 Regulates DLG2, PP2A, VCP, EB1, and Ankrd26.
Tumor Suppressor RARRES1 Regulates DLG2, PP2A, VCP, EB1, and Ankrd26.
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DOI：
10.7150/jca.1.14
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发表时间：
2010-06-02
影响因子：
3.9
通讯作者：
Byers SW
中科院分区：
文献类型：
--
作者：
Sahab ZJ;Hall MD;Zhang L;Cheema AK;Byers SW
视黄酸受体应答基因（RARRES 1）是一种功能未知的肿瘤抑制基因，最初被鉴定为皮肤中的一种新的视黄酸受体调控基因。RARRES 1在永生化的人前列腺上皮细胞系PWR-1 E细胞中被敲低，并且使用差异凝胶电泳（DIGE）随后基质辅助激光解吸电离（MALDI）质谱和蛋白质印迹分析来鉴定差异蛋白质表达，排除了几乎所有蛋白质组学项目中常规鉴定的高度丰富的蛋白质。RARRES 1的敲除：1-下调PP 2A，一种参与生长激素刺激的信号转导途径的负调节的酶; 2-下调导致受损的自噬的含瓦洛辛的蛋白质; 3-上调肿瘤抑制盘大2; 4-上调属于POTE家族基因的Ankrd 26，其在具有不良结果的癌症患者中高度表达; 5-下调EB 1，一种在有丝分裂过程中参与纺锤体动力学和染色体排列的蛋白质。
Retinoic Acid Receptor Responder (RARRES1) initially identified as a novel retinoic acid receptor regulated gene in the skin is a putative tumor suppressor of unknown function. RARRES1 was knocked down in immortalized human prostatic epithelial cell line PWR-1E cells and differential protein expression was identified using differential in-gel electrophoresis (DIGE) followed by matrix-assisted laser desorption ionization (MALDI) mass spectrometry and western Blot analysis excluding highly abundant proteins routinely identified in almost all proteomics projects. Knock-down of RARRES1: 1- down-regulates PP2A, an enzyme involved in the negative regulation of the growth hormone-stimulated signal transduction pathways; 2- down-regulates Valosin-containing protein causing impaired autophagy; 3- up-regulates the tumor suppressor disks large 2; 4- up-regulates Ankrd26 that belongs to the POTE family of genes that are highly expressed in cancer patients with poor outcome; and 5- down-regulates EB1, a protein that is involved in spindle dynamics and chromosome alignment during mitosis.