C. Cell Proliferation Kinetics Analyzed with BrdU and Near-UV Light Treatment1
C. Cell Proliferation Kinetics Analyzed with BrdU and Near-UV Light Treatment1
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C. 使用 BrdU 和近紫外光处理分析细胞增殖动力学1
DOI：
10.1159/000408411
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
M. Hagan
中科院分区：
文献类型：
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作者：
M. Hagan
自1957年Greer和Zamenhof [11]发现以来，含有溴脱氧尿苷（BrdU）取代DNA的细胞对紫外线（UV）的敏化已被证明是分子生物学家的有力工具。用BrdU取代的DNA的光解为诸如突变体选择[1]、基因作图[2]和DNA修复“补丁长度”分析[20]等多种应用提供了技术。此外，BrdU取代DNA的质量增加已经形成了复制保护性复制[9]和DNA修复复制[24，30]研究的基础。BrdU的第三个特性，即与各种荧光染料的相互作用，提高了姐妹染色单体交换分析的灵敏度[15，18]。后一种技术还提供了有关体外细胞周期动力学的重要信息[4，8]。虽然BrdU已经显示出这些体外测定的优点，但其在体内的使用仅显示出有限的成功。体内缺乏成功的主要原因是BrdU在宿主中的快速分解[16，17]。
Since its discovery by Greer and Zamenhof [11] in 1957, the sensitization to ultraviolet (UV) light of cells containing bromodeoxyuridine (BrdU)-substituted DNA has proven to be a powerful tool for the molecular biologist. The photolysis of DNA substituted with BrdU has provided techniques for such diverse applications as mutant selection [1], gene mapping [2], and DNA repair'patch-length'analysis [20]. Also, the increased mass of the BrdU-substituted DNA has formed the basis for studies of semiconservative replication [9] and DNA repair replica-tion [24, 30]. A third property of BrdU, its interaction with various fluo-rescent dyes, has yielded increased sensitivity for the analysis of sister chromatid exchange [15, 18]. The latter technique has also provided con-siderable information concerning in vitro cell cycle kinetics [4, 8]. Although BrdU has shown merit for these in vitro assays, its use in vivo has shown only limited success. The primary reason for this lack of success in vivo is the rapid breakdown of BrdU in the host [16, 17].