Construction of energy transfer systems within nanosized polymer micelles and their fluorescence modulation properties.
Construction of energy transfer systems within nanosized polymer micelles and their fluorescence modulation properties.
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DOI：
10.1002/cphc.200900999
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发表时间：
2010-04
期刊：
影响因子：
--
通讯作者：
Jian Chen;Fang Zeng;Shuizhu Wu
中科院分区：
文献类型：
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作者：
Jian Chen;Fang Zeng;Shuizhu Wu
基于纳米颗粒的荧光共振能量转移（FRET）系统由于其一些优点最近引起了广泛的关注；然而，这些系统在结构上比小分子系统更复杂，并且粒子系统的能量传递行为很少被研究。在本研究中，我们通过连续两步原子转移合成了一系列包含亲水性聚环氧乙烷（PEO）、疏水性聚苯乙烯（PS）和光致变色聚（2-（3-（3',3'-二甲基-6-硝基螺（indoline-2',2-[2H-1]苯并吡喃）-1'-基）丙酰氧基）甲基丙烯酸乙酯）（PSPMA）嵌段的ABC三嵌段共聚物自由基聚合（ATRP）。由这些共聚物制成的两亲性胶束可以掺入疏水性荧光染料硝基苯并恶二唑基衍生物（NBD），从而形成以荧光染料NBD为供体、螺吡喃部分为潜在受体的水分散性能量转移系统。研究了具有不同PS嵌段长度和PSPMA嵌段长度的NBD/三嵌段共聚物的光谱特性；结果表明，三嵌段共聚物中PS嵌段的长度会影响基于胶束的荧光调制系统的能量转移效率，中等PS嵌段长度的胶束有利于形成具有较高能量转移效率的FRET系统，而短PSPMA嵌段（少于5个重复单元）足以实现高效的能量转移。
The nanoparticle-based fluorescence resonance energy transfer (FRET) systems have recently attracted considerable attention due to some of their advantages; however, these systems are structurally more complicated than that of small molecule systems, and the energy transfer behavior for particle systems has been seldom investigated. In this study, we synthesized a series of ABC triblock copolymers that contain hydrophilic poly(ethylene oxide) (PEO), hydrophobic poly(styrene) (PS) and photochromic poly(2-(3-(3',3'-dimethyl-6-nitrospiro(indoline-2',2-[2H-1]benzopyran)-1'-yl)propanoylo-xy)ethyl methacrylate) (PSPMA) blocks by using the sequential two-step atom transfer radical polymerization (ATRP). The amphiphilic micelles made from these copolymers can incorporate a hydrophobic fluorescent dye, the nitrobenzoxadiazolyl derivative (NBD), thus forming a water dispersible energy transfer system with the fluorescent dye NBD as the donor and spiropyran moieties as the potential acceptor. The spectral properties of NBD/triblock copolymers with varied PS block lengths and PSPMA block lengths have been investigated; the results indicate that the length of PS block in triblock copolymers can affect the energy transfer efficiency of the micelle-based fluorescence modulation system, the micelles with moderate PS block length are preferable for forming FRET system with higher energy transfer efficiency, and short PSPMA blocks (less than 5 repetition units) are enough for efficient energy transfer.