Xenopus suppressor of Hairless 2 is involved in the cell fate decision during gastrulation through the transcriptional regulation of Xoct25/91
Xenopus suppressor of Hairless 2 is involved in the cell fate decision during gastrulation through the transcriptional regulation of Xoct25/91
复制标题
DOI：
10.1016/j.bbrc.2006.12.087
复制
发表时间：
2007-02-16
影响因子：
3.1
通讯作者：
Kinoshita, Tsutomu
中科院分区：
文献类型：
--
作者：
Ito, Motoaki;Nishitani, Eriko;Kinoshita, Tsutomu
我们先前已经表明，非洲爪蟾无毛抑制因子2，XSu（H）2，参与原肠胚胚胎的形态发生，从XESR-1介导的Notch信号通路的不同方式。为了解决XSu（H）2的下游因素，我们研究了XSu（H）2对非洲爪蟾PO U-V基因Xoct 25和Xoct 91的影响。敲低XSu（H）2可导致Xoct 25、Xoct 91和Xbrachyury表达下调。显性负性Xoct 25导致囊胚孔关闭延迟，同时Xbrachyury表达降低。Xoct 25或Xoct 91的过表达可以挽救由XSu（H）2缺失引起的Xbrachyury表达的降低。XSu（H）2 EnR抑制CHX处理的动物帽中Xoct 25和Xoct 91的表达。Xoct 25的启动子分析表明，Xoct 25的上游区域含有预期的Su（H）结合基序，这是Xoct 25转录所必需的。这些结果表明，XSu（H）2通过Xoct 25/91介导的途径的基因表达参与原肠胚形成期间的细胞命运决定。(c)2006年爱思唯尔公司All rights reserved.
We have previously indicated that Xenopus Suppressor of Hairless 2, XSu(H)2, is involved in the morphogenesis of gastrula embryos in a different manner from the XESR-1-mediated Notch signaling pathway. To address the downstream factors of XSu(H)2, we investigated the effect of XSu(H)2 on Xenopus PO U-V genes, Xoct25 and Xoct91. Knockdown of XSu(H)2 caused the downregulation of Xoct25, Xoct91 and Xbrachyury. Dominant-negative Xoct25 caused the delay of blastopore closure with a decrease of Xbrachyury expression. Overexpression of Xoct25 or Xoct91 could rescue the decrease of Xbrachyury expression caused by XSu(H)2 depletion. XSu(H)2EnR inhibited Xoct25 and Xoct91 expressions in CHX-treated animal caps. Promoter analysis of the Xoct25 showed that the upstream region of Xoct25 contains the prospective Su(H) binding motif, which is essential for the transcription of Xoct25. These results suggest that XSu(H)2 is engaged in the cell fate decision during gastrulation through the gene expression of the Xoct25/91-mediated pathway. (c) 2006 Elsevier Inc. All rights reserved.