Altered circulating hormone levels, endothelial function and vascular reactivity in the testicular feminised mouse
Altered circulating hormone levels, endothelial function and vascular reactivity in the testicular feminised mouse
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DOI：
10.1530/eje.0.1480111
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发表时间：
2003-01-01
影响因子：
5.8
通讯作者：
Jones, TH
中科院分区：
文献类型：
--
作者：
Jones, RD;Pugh, PJ;Jones, TH
目的：睾丸雌性化（Tfm）小鼠表达无功能雄激素受体，并且循环睾酮水平降低。最近的研究支持睾酮的心脏保护作用，因为它增强全身和肺血管舒张。本研究的目的是确定是否雄激素不敏感和低睾酮血症的TFM小鼠与异常血管反应性或激素status.Methods：成年雄性TFM和同窝对照小鼠被杀死，收集血液。解剖股动脉（直径范围= 183-508 μ m）和肺动脉（直径范围= 320-816 μ m），并分别以100 mmHg或17.5 mmHg加载到钢丝或压力肌描记器中。氯化钾血管反应性的药理学评估（KCl，8 0 mmol/l）和去甲肾上腺素（NA，1 nmol/1-100 mumol/l）和乙酰胆碱（ACh，0.1-100 μ mol/l）或睾酮（1 nmol/1-100 mumol/l）。Tfm小鼠的睾丸激素水平降低（1.8+/-0.3 nmol/l）与对照组相比（9.3 ± 2.0 nmol/l，P < 0.001）和胆固醇水平升高（3.6 ± 0.1 mmol/l，P < 0.05）。Tfm小鼠的股动脉在KCl浓度为80 mmol/l时的血管收缩（3.27+/-0.23 mN/mm）较对照组（4.44+/-0.41 mN/mm，P
Objective: Testicular feminised (Tfm) mice express a non-functional androgen receptor, and also have reduced levels of circulating testosterone. Recent studies support a cardio-protective role for testosterone since it elicits systemic and pulmonary vasodilatation. The aim of the present study was to determine whether androgen insensitivity and hypotestosteronaemia in the Tfm mouse are associated with abnormal vascular reactivity or hormone status.Methods: Adult male Tfm and littermate control mice were killed and the blood collected. Femoral (diameter range = 183-508 mum) and pulmonary (diameter range = 320-816 mum) arteries were dissected and loaded in either a wire or pressure myograph, at 100 mmHg or 17.5 mmHg respectively. Pharmacological assessment of the vasoreactivity to potassium chloride (KCl, 8 0 mmol/l) and either noradrenaline (NA, 1 nmol/1-100 mumol/l) and acetylcholine (ACh, 0.1-100 mumol/l) or testosterone (1 nmol/1-100 mumol/l) was then made.Results: Tfm mice had reduced levels of testosterone (1.8+/-0.3 nmol/l) compared with controls (9.3+/-2.0 nmol/l, P < 0.001) and elevated levels of cholesterol (3.6+0.1 mmol/l) compared with controls (3.2+/-0.1 mmol/l, P < 0.05). Femoral arteries from Tfm mice exhibited reduced vasoconstriction to 80 mmol/l KCl (3.27+/-0.23 mN/mm) compared with vessels from controls (4.44+/-0.41 mN/mm, P