Enamel matrix derivative (EMD) enhances the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs).
Enamel matrix derivative (EMD) enhances the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs).
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牙釉质基质衍生物（EMD）增强骨髓间充质干细胞（BMSC）的成骨分化
DOI：
10.1080/21655979.2021.1971504
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发表时间：
2021-12
期刊：
影响因子：
4.9
通讯作者：
Dong Q
中科院分区：
文献类型：
--
作者：
Cheng L;Li Y;Xia Q;Meng M;Ye Z;Tang Z;Feng H;Chen X;Chen H;Zeng X;Luo Y;Dong Q
探讨骨髓间充质干细胞向成骨分化的能力。用EMD处理BMSCs，扫描电子显微镜和茜素红染色检测BMSCs在体内和体外成骨能力的变化，并用CCK8评价BMSCs的增殖能力。此外，通过加入Wnt/β-catenin信号通路的典型抑制剂XAV939，阐明了Wnt/β-catenin信号的调节功能。结果表明，EMD对细胞增殖有促进作用，其中以25μg/mlEMD的促进作用最为显著。诱导成骨细胞2、3甚至4周后，茜素红染色显示EMD组细胞染色较对照组深(P<0.05)，提示BMSCs矿化程度较高。将包被25μg/mlEMD处理的骨髓间充质干细胞膜包裹的钛板植入裸鼠体内8周后，钛板表面形成的结节比对照组多(P<0.05)。他显示，有少许蓝紫色未成熟的骨样组织块。此外，XAV939组RUNX家族转录因子2、Osterix、骨钙素、I型胶原、碱性磷酸酶和β-catenin的表达均受到抑制(P<0.05)，而EMD组则均被激活(P<0.05)。综上所述，EMD促进了BMSCs的增殖和成骨分化。EMD对骨髓间充质干细胞的作用可能与Wnt/β-catenin信号通路有关。
To investigate the EMD’s capacity in BMSCs osteogenic differentiation. In vivo and in vitro, BMSCs were treated with EMD, scanning electron microscopy, and Alizarin Red staining were used to detect the changes in the osteogenic ability of BMSCs, and the proliferation ability of BMSCs was evaluated by CCK8. In addition, by adding xav939, a typical inhibitor of Wnt/β-catenin signaling pathway, the regulatory function of Wnt/β-catenin signaling was clarified. The results showed that EMD promote cell proliferation and 25 μg/ml EMD had the most significant effect. Cells inducing osteogenesis for 2 and 3 even 4 weeks, the cell staining is deeper in EMD treated group than that of the control (P < 0.05) by alizarin Red staining, suggesting more mineralization of BMSCs. In vivo implanting the titanium plate wrapped with 25 μg/ml EMD treated-BMSC film into nude mice for 8 weeks, more nodules were formed on the surface of the titanium plate than that the control (P < 0.05). HE showed that there is a little blue-violet immature bone-like tissue block. Besides, the expression of RUNX Family Transcription Factor 2 (Runx2), Osterix, Osteocalcin (OCN), collagen I (COLI), alkaline phosphatase (ALP) and β-catenin were inhibited in xav939 group (P < 0.05); Inversely, all were activated in EMD group (P < 0.05). In conclusion, EMD promoted the proliferation and osteogenic differentiation of BMSCs. EMD’s function on BMSCs might be associated with the Wnt/β-catenin signaling pathway.
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影响因子：
14
作者：
Kémoun P;Gronthos S;Snead ML;Rue J;Courtois B;Vaysse F;Salles JP;Brunel G
通讯作者：
Brunel G
DOI：
10.3803/enm.2018.33.3.318
发表时间：
2018-09
期刊：
Endocrinology and metabolism (Seoul, Korea)
影响因子：
--
作者：
Moorer MC;Riddle RC
通讯作者：
Riddle RC
影响因子：
9.3
作者：
Gu, Qiuhan;Chen, Chen;Shi, Lei
通讯作者：
Shi, Lei
影响因子：
4.8
作者：
Artigas, Natalia;Urena, Carlos;Ventura, Francesc
通讯作者：
Ventura, Francesc
影响因子：
5.8
作者：
Apicella, Alessandra;Heunemann, Peggy;Fischer, Peter
通讯作者：
Fischer, Peter