Intraepithelial lymphocytes express junctional molecules in murine small intestine
Intraepithelial lymphocytes express junctional molecules in murine small intestine
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DOI：
10.1016/j.bbrc.2005.04.025
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发表时间：
2005-06-17
影响因子：
3.1
通讯作者：
Nawa, Y
中科院分区：
文献类型：
--
作者：
Inagaki-Ohara, K;Sawaguchi, A;Nawa, Y
肠道上皮内淋巴细胞（intraepithelial lymphocytes，IEL）位于基底外侧，调节上皮细胞（epithelial cells，EC）的增殖和分化，是宿主肠道防御的第一道防线。然而，它仍然是未知的IEL如何互动和沟通EC。在这里，我们表明IEL表达连接分子，如EC。我们鉴定了IEL中功能分子的mRNA表达，如紧密连接蛋白（ZO）-1、闭合蛋白和连接粘附分子（JAM）（紧密连接）、β-连环蛋白和E-钙粘蛋白（粘附连接）以及间隙连接蛋白26（间隙连接）。IEL在蛋白水平上组成性表达occludin和E-cadherin，而胸腺、脾脏、肝脏、肠系膜淋巴结和派尔集合淋巴结中的其他T细胞则不表达。γ δ IEL的表达水平高于α β IEL。occludin的表达增加抗CD 3抗体刺激。这些结果表明，一个新的作用IEL有关上皮屏障和IEL和EC之间的通信的可能性。(c)2005年爱思唯尔公司All rights reserved.
Intestinal intraepithelial lymphocytes (IEL) that reside at basolateral site regulate the proliferation and differentiation of epithelial cells (EC) for providing a first line of host defense in intestine. However, it remains unknown how IEL interact and communicate with EC. Here, we show that IEL express junctional molecules like EC. We identified mRNA expression of the functional molecules in IEL such as zonula occludens (ZO)-1, occludin and junctional adhesion molecule (JAM) (tight junction), beta-catenin and E-cadherin (adherens junction), and connexin26 (gap junction). IEL constitutively expressed occludin and E-cadherin at protein level, while other T cells in the thymus, spleen, liver, mesenteric lymph node, and Peyer's patches did not. gamma delta IEL showed higher level of these expressions than alpha beta IEL. The expression of occludin was augmented by anti-CD3 Ab stimulation. These results suggest the possibility of a novel role of IEL concerning epithelial barrier and communication between IEL and EC. (c) 2005 Elsevier Inc. All rights reserved.