The action of piracetam on the electrical activity of the hippocampal slice preparation: a field potential analysis.
The action of piracetam on the electrical activity of the hippocampal slice preparation: a field potential analysis.
复制标题
吡拉西坦对海马切片电活动的作用：场电位分析。
DOI：
10.1016/0014-2999(82)90088-7
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发表时间：
1982
影响因子：
5
通讯作者：
Lynch,GS
中科院分区：
文献类型：
--
作者：
Olpe,HR;Lynch,GS
研究了不同剂量吡拉西坦对大鼠离体海马片电反应的影响。吡拉西坦剂量依赖性地增加了辐射层刺激引起的锥体神经元群体尖峰反应的幅度。吡拉西坦在剂量为100 μM时开始影响种群峰值，并在剂量为1 mM时持续增加。该药物起效迅速，在移除后几分钟内即可恢复。浓度高达50 mM的吡拉西坦不影响锥体神经元对反向刺激的反应。此外，药物既不会改变树突反应的振幅，也不会改变树突反应的斜率。吡拉西坦也不影响破伤风后或突触电位的长期增强。这些发现表明，该药物并不通过锥体细胞的去极化或位于其树突上的突触过程的增强而起作用。通过一系列实验研究了吡拉西坦对篮细胞前馈和反馈抑制锥体细胞的作用。在浓度为1和10 mM时，吡拉西坦没有改变两种类型的抑制机制。讨论了吡拉西坦的几种假设作用位点。
The action of various doses of piracetam on the electrical responses of in vitro hippocampal slices from the rat was investigated. Piracetam increased dose dependently the amplitude of the population spike response of pyramidal neurons evoked by stimulation of the stratum radiatum. Piracetam began to affect the population spike at a dose of 100 μM and consistently increased it at a dose of 1 mM. The drug had a rapid onset of action and recovery was seen within a few minutes following its removal. The response of the pyramidal neurons to antidromic stimulation was not affected by piracetam at concentrations up to 50 mM. Also, neither the amplitude nor the slope of the dendritic response was changed by the drug. Piracetam also did not affect either posttetanic or long-term potentiation of synaptic potentials. These findings suggest that the drug does not act through depolarization of the pyramidal cells or potentiation of the synaptic processes located on their dendrites. The action of piracetam on the feedforward- and feedback-mediated inhibition of pyramidal cells by basket cells was investigated in several series of experiments. At concentrations of 1 and 10 mM, piracetam did not change either of the two types of inhibitory mechanisms. Several hypothetical sites of action of piracetam are discussed.