Adipose tissue engineering using mesenchymal stem cells attached to injectable PLGA spheres
Adipose tissue engineering using mesenchymal stem cells attached to injectable PLGA spheres
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DOI：
10.1016/j.biomaterials.2005.02.022
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发表时间：
2005-10-01
期刊：
影响因子：
14
通讯作者：
Suh, H
中科院分区：
文献类型：
--
作者：
Choi, YS;Park, SN;Suh, H
软组织缺损的重建仍然是整形和重建手术中的一个挑战，并且临床上确实需要适当的解决方案。进行这项研究的目的是为了将间充质干细胞 (MSC) 分化为脂肪细胞，然后评估通过将 MSC 附着到可注射的 PLGA 球体来构建脂肪组织的可能性。我们还设计了可注射的 PLGA 球体，用于无疤痕移植。在本研究中，将MSCs和adipo-MSCs（在成脂培养基中培养7天的MSCs）附着在PLGA球上并培养7天，然后注射到裸鼠体内2周。结果，在体外，第 1 天和第 7 天脂肪-间充质干细胞中脂质积累的差异比间充质干细胞中的要高得多。注射两周后，APLGA组形成大量新组织，而MPLGA组仅形成少量。我们通过 GFP 检测验证了新形成的组织源自注射的 MSC，并通过油红 O 染色和 Western blot 确认所创建的组织是真正的脂肪组织（PPAR γ 和 C/EBP α 仅在 APLGA 组中表达）。因此，本研究提出了一种使用 MSC 和可注射 PLGA 球体进行脂肪组织工程的有效模型。 (c) 2005 Elsevier Ltd. 保留所有权利。
The reconstruction of soft tissue defects remains a challenge in plastic and reconstructive surgery, and a real clinical need exists for an adequate solution. This study was undertaken in order to differentiate mesenchymal stem cells (MSCs) into adipocytes, and to then assess the possibility of constructing adipose tissue via the attachment of MSCs to injectable PLGA spheres. We also designed injectable PLGA spheres for scar-free transplantation. In this study, MSCs and adipo-MSCs (MSCs cultured in adipogenic medium for 7 days) were attached to PLGA spheres and cultured for 7 days, followed by injection into nude mice for 2 weeks. As a result, the difference between lipid accumulation in adipo-MSCs at 1 and 7 days was much higher in vitro than in the MSCs. Two weeks after injection, a massive amount of new tissue was formed in the APLGA group, whereas only a small amount was formed in the MPLGA group. We verified that the newly formed tissue originated from the injected MSCs via GFP testing, and confirmed that the created tissue was actual adipose tissue by oil red O staining and Western blot (PPAR gamma and C/EBP alpha were expressed only in APLGA groups). Therefore, this study presents an efficient model of adipose tissue engineering using MSCs and injectable PLGA spheres. (c) 2005 Elsevier Ltd. All rights reserved.