Orientated Guidance of Peripheral Nerve Regeneration Using Conduits with a Microtube Array Sheet (MTAS).
Orientated Guidance of Peripheral Nerve Regeneration Using Conduits with a Microtube Array Sheet (MTAS).
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DOI：
10.1021/acsami.5b00215
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发表时间：
2015-04
影响因子：
9.5
通讯作者：
Yueming Wang;Wen-Jin Wang;Yan Wo;Ting Gui;Hao Zhu;X. Mo;C. Chen;Qingfeng Li;W. Ding
中科院分区：
文献类型：
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作者：
Yueming Wang;Wen-Jin Wang;Yan Wo;Ting Gui;Hao Zhu;X. Mo;C. Chen;Qingfeng Li;W. Ding
材料表面的表面形态在体外可以影响细胞的生物学行为；然而，在体内对周围神经再生的影响还没有被探索。在这里，我们研究了一种具有独特纵向表面形貌的微管阵列片(MTAS)在体内和体外促进周围神经再生效率的潜力。将雪旺细胞、脊髓运动神经元和背根神经节神经元接种于MTAS上，研究其对神经细胞生物学特性和行为的影响。MTAS引导雪旺细胞的定向迁移，而不影响其他关键的生物学特性，如增殖和神经营养素的表达。此外，MTAS还指导了两种类型神经元的神经突起的定向延伸。接下来，我们测试了MTAS通过用装有MTAS衬里的神经导管桥接大鼠10 mm坐骨神经缺损来促进周围神经再生的能力。MTAS显著促进周围神经再生，导管中段的纤维口径较大，导管远端神经段的纤维较丰富。扫描电子显微镜分析表明MTAS对神经再生有定向引导作用，MTAS组神经纤维偏心率较小，胶原纤维排列整齐。我们的研究结果表明，本研究开发的MTAS导管具有显著的潜力，可以通过改变关键的生物学行为和指导定向神经生长来促进周围神经再生。
Material surface topography has been shown to affect the biological behavior of cells in vitro; however, the in vivo effect on peripheral nerve regeneration has not been explored. Here, we studied the potential of a microtube array sheet (MTAS) with a unique longitudinal surface topography to promote peripheral nerve regeneration efficiency, both in vivo and in vitro. Schwann cells, spinal cord motor neurons, and dorsal root ganglion neurons were seeded on the MTAS to study the effect of the construct on the biological properties and behaviors of neural cells. The MTAS guided the oriented migration of Schwann cells without affecting other critical biological properties, such as proliferation and neurotrophin expression. In addition, the MTAS guided the directed extension of neurites from both types of neurons. Next, we tested the capability of the MTAS to facilitate peripheral nerve regeneration by bridging a 10 mm sciatic nerve defect in rats with a nerve conduit equipped with an MTAS lining. The MTAS significantly promoted peripheral nerve regeneration, as suggested by the greater fiber caliber in the midconduit and the greater abundance of fibers in nerve segment distal to the conduit. Moreover, scanning electron microscopy (SEM) analysis suggested the orientated guidance of nerve regeneration by the MTAS, as indicated by the smaller eccentricity of the nerve fibers and the concordant arrangement of the collagen fiber in both the fibers and the matrix in the MTAS group. Our results collectively suggest that the conduits with the MTAS developed in this study have significant potential for facilitating peripheral nerve regeneration by modifying critical biological behaviors and guiding orientated nerve growth.