β2 Adrenergic receptor activation suppresses BMP-induced alkaline phosphatase expression in osteoblast-like MC3T3E1 cells.
β2 Adrenergic receptor activation suppresses BMP-induced alkaline phosphatase expression in osteoblast-like MC3T3E1 cells.
复制标题
β2 肾上腺素受体激活抑制成骨细胞样 MC3T3E1 细胞中 BMP 诱导的碱性磷酸酶表达。
DOI：
10.1002/jcb.25071
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发表时间：
2015
影响因子：
4
通讯作者：
M.
中科院分区：
文献类型：
--
作者：
Yamada;T.;Ezura;Y.;Hayata;T.;Moriya;S.;Shirakawa;J.;Notomi;T.;Arayal;S.;Kawasaki;M.;Izu;Y.;Harada;K.;and Noda;M.
β肾上腺素能刺激在体内抑制骨形成，但其在成骨细胞分化中的作用尚不完全清楚。因此，我们研究了骨形态发生蛋白2肾上腺素能刺激对成骨细胞样MC3T3E1细胞的影响，重点是β诱导的碱性磷酸酶的表达。在形态上，异丙肾上腺素可抑制BMP诱导的MC3T3-E1细胞培养中碱性磷酸酶阳性小病灶数量的增加。在生化方面，异丙肾上腺素处理以剂量依赖的方式抑制BMP诱导的碱性磷酸酶的活性。即使用高浓度的BMP处理细胞，异丙肾上腺素也能抑制碱性磷酸酶的活性。在细胞密度方面，在高密度培养的成骨细胞中，异丙肾上腺素处理更倾向于抑制BMP诱导的碱性磷酸酶表达的增加。在治疗方案方面，异丙肾上腺素连续治疗与周期治疗相比较。持续异丙肾上腺素治疗对BMP诱导的碱性磷酸酶表达增加的抑制作用比周期治疗更明显。在分子水平上，异丙肾上腺素可抑制BMP诱导的碱性磷酸酶基因表达的增强。关于异丙肾上腺素的作用方式，异丙肾上腺素抑制BMP诱导的bre-荧光素酶活性。这些数据表明，异丙肾上腺素调节BMP诱导的成骨细胞样MC3T3E1细胞碱性磷酸酶的表达。J.细胞。生物化学。2015年116：1144-1152。©2014 Wiley期刊，Inc.
β adrenergic stimulation suppresses bone formation in vivo while its actions in osteoblastic differentiation are still incompletely understood. We therefore examined the effects of β2adrenergic stimulation on osteoblast‐like MC3T3‐E1 cells focusing on BMP‐induced alkaline phosphatase expression. Morphologically, isoproterenol treatment suppresses BMP‐induced increase in the numbers of alkaline phosphatase‐positive small foci in the cultures of MC3T3‐E1 cells. Biochemically, isoproterenol treatment suppresses BMP‐induced enzymatic activity of alkaline phosphatase in a dose‐dependent manner. Isoproterenol suppression of alkaline phosphatase activity is observed even when the cells are treated with high concentrations of BMP. With respect to cell density, isoproterenol treatment tends to suppress BMP‐induced increase in alkaline phosphatase expression more in osteoblasts cultured at higher cell density. In terms of treatment protocol, continuous isoproterenol treatment is compared to cyclic treatment. Continuous isoproterenol treatment is more suppressive against BMP‐induced increase in alkaline phosphatase expression than cyclic regimen. At molecular level, isoproterenol treatment suppresses BMP‐induced enhancement of alkaline phosphatase mRNA expression. Regarding the mode of isoproterenol action, isoproterenol suppresses BMP‐induced BRE‐luciferase activity. These data indicate that isoproterenol regulates BMP‐induced alkaline phosphatase expression in osteoblast‐like MC3T3E1 cells. J. Cell. Biochem. 116: 1144–1152, 2015. © 2014 Wiley Periodicals, Inc.