Inflammatory changes in adipose tissue enhance expression of GPR84, a medium-chain fatty acid receptor TNFα enhances GPR84 expression in adipocytes
Inflammatory changes in adipose tissue enhance expression of GPR84, a medium-chain fatty acid receptor TNFα enhances GPR84 expression in adipocytes
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DOI：
10.1016/j.febslet.2012.01.001
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发表时间：
2012-02-17
期刊：
影响因子：
3.5
通讯作者：
Hamada, Yoji
中科院分区：
文献类型：
--
作者：
Nagasaki, Hiroshi;Kondo, Takaaki;Hamada, Yoji
在这项研究中，我们的目的是确定G蛋白偶联受体84（GPR 84）在脂肪组织中的生理作用，以及中链脂肪酸（MCFA），GPR 84的特异性配体。在小鼠中，高脂饮食上调了脂肪垫中的GPR 84表达。在3 T3-L1脂肪细胞中，与巨噬细胞系RAW 264或TNF α共培养显著增强GPR 84表达。在TNF α存在下，MCFA下调3 T3-L1脂肪细胞中脂联素mRNA的表达。综上所述，我们的结果表明，GPR 84出现在脂肪细胞中，以响应来自浸润巨噬细胞的TNF α，并加剧了肥胖和糖尿病之间的恶性循环。(C)2012年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
In this study we aimed to identify the physiological roles of G protein-coupled receptor 84 (GPR84) in adipose tissue, together with medium-chain fatty acids (MCFAs), the specific ligands for GPR84. In mice, high-fat diet up-regulated GPR84 expression in fat pads. In 3T3-L1 adipocytes, co-culture with a macrophage cell line, RAW264, or TNF alpha remarkably enhanced GPR84 expression. In the presence of TNF alpha, MCFAs down-regulated adiponectin mRNA expression in 3T3-L1 adipocytes. Taken together, our results suggest that GPR84 emerges in adipocytes in response to TNF alpha from infiltrating macrophages and exacerbates the vicious cycle between adiposity and diabesity. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.