High amounts of nuclear DNA in tomato (Lycopersicon esculentum Mill) pericarp
High amounts of nuclear DNA in tomato (Lycopersicon esculentum Mill) pericarp
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DOI：
10.1016/0168-9452(96)04383-x
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发表时间：
1996-05-03
期刊：
影响因子：
5.2
通讯作者：
Bino, RJ
中科院分区：
文献类型：
--
作者：
Bergervoet, JHW;Verhoeven, HA;Bino, RJ
在番茄果实发育过程中，测定了果皮的DNA合成。分离的细胞核用DAPI染色后，用流式细胞仪分析了DNA的相对含量。结果表明，在年轻的绿色果实中，C值与发育中的叶片相当，即主要是具有2C和4C DNA含量的细胞，其中C是生殖系细胞基因组的DNA含量单位[1]。在番茄果实的进一步发育过程中，大多数细胞转变为更高的C值（高达256 C），组织变得高度多体细胞。这些结果与用激光共聚焦扫描显微镜测量果皮组织的核直径获得的数据相关。核直径在果实发育期间增加约6倍，在红熟果实中达到最大值。
During tomato fruit development the DNA synthesis of the pericarp was measured, After staining of the isolated nuclei with DAPI the relative DNA amount was analyzed using a flow cytometer. Results showed that in the young green fruit the C-values are comparable with those of developing leaves i.e. predominantly cells with 2C and 4C DNA content, where C is the unit of DNA content of the genome of germ-line cells [1]. During further tomato fruit development most of the cells switch to higher C-values (up to 256C) and the tissue becomes highly polysomatic. These results are correlated to the data obtained by measuring the diameter of the nuclei of the pericarp tissue with a confocal scanning laser microscope, The diameter of the nuclei increased during fruit development similar to 6 times reaching a maximum in the red ripe fruit.