pH-controllable drug carrier with SERS activity for targeting cancer cells
pH-controllable drug carrier with SERS activity for targeting cancer cells
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pH可控的药物载体，具有SERS活性，可靶向癌细胞。
DOI：
10.1016/j.bios.2014.01.042
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发表时间：
2014-07-15
影响因子：
12.6
通讯作者：
Cui, Yiping
中科院分区：
文献类型：
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作者：
Fang, Wei;Wang, Zhuyuan;Cui, Yiping
基于介孔二氧化硅纳米颗粒和壳聚糖/聚甲基丙烯酸制备了一种pH可控的药物载体，可同时作为靶向癌细胞的表面增强拉曼散射(SERS)示踪药物载体。将壳聚糖/聚甲基丙烯酸(CS-PMAA)包覆在纳米粒子表面，可以获得对pH敏感的释药特性，而核中标记了SERS的银纳米粒子可以获得较强的SERS信号。我们的实验结果表明，阿霉素(DOX)被有效地包裹在纳米载体中，并可以随着环境pH值的变化而释放。具体地说，在较低的pH值下，DOX的释放量增加。此外，将复合纳米粒与转铁蛋白(Tf)偶联，以靶向转铁蛋白受体(TfR)过表达的癌细胞。通过SERS图谱研究药物载体的靶向能力和胞内定位，通过荧光图像监测DOX的分布。结果表明，所制备的药物载体可以同时实现pH响应性药物释放、SERS示踪和肿瘤细胞靶向功能，具有独特的pH可控药物释放纳米系统的潜力。(C)2014爱思唯尔B.V.保留所有权利。
A type of pH-controllable drug carrier is demonstrated based on mesoporous silica nanoparticles and chitosan/poly (methacrylic acid), which can simultaneously serve as the surface enhanced Raman scattering (SERS) traceable drug carriers for targeting cancer cells. The pH-sensitive releasing characteristics can be achieved by coating the nanoparticles with a layer of chitosan/poly (methacrylic acid) (CS-PMAA), while strong SERS signals can be obtained from the SERS reporter tagged Ag nanoparticles in the core. Our experimental results show that doxorubicin (DOX) was effectively encapsulated into the nanocarriers and can be released in response to the ambient pH value. Specifically, an increased amount of DOX release was observed at lower pH value. In addition, the composite nanoparticles were conjugated with transferrin (Tf) to target transferrin receptor (TfR)-overexpressed cancer cells. The targeting ability as well as the intracellular location of the drug carrier was investigated through SERS mapping while the distribution of DOX was monitored by fluorescence images. The results show that the demonstrated drug carrier can simultaneously fulfill the functionalities of pH-responsive drug release, SERS-traceable characteristics and cancer cells targeting, which has a unique potential for the pHcontrollable drug delivery nanosystems. (C) 2014 Elsevier B.V. All rights reserved.