Activation of the Non-canonical Inflammasome in Mouse and Human Cells.
Activation of the Non-canonical Inflammasome in Mouse and Human Cells.
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小鼠和人类细胞中非典型炎症体的激活。
DOI：
10.1007/978-1-0716-2144-8_5
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Bezbradica JS
中科院分区：
文献类型：
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作者：
Bezbradica JS
非典型炎性体是一个信号平台，允许检测免疫和非免疫细胞中的细胞质脂多糖（LPS）。检测到LPS后，该炎性体激活信号蛋白酶caspase-4和-5（在人类中）和caspase-11（在小鼠中）。炎症性半胱天冬酶激活导致半胱天冬酶自我加工和成孔蛋白Gasdermin D （GSDMD）的裂解。GSDMD n端片段寡聚并在质膜上形成孔，导致炎症形式的细胞死亡，称为焦亡。在这里，我们描述了一种简单的方法来激活髓细胞和上皮细胞中的非典型炎性体，并使用细胞死亡试验和免疫印迹法测量其活性。
The non-canonical inflammasome is a signaling platform that allows for the detection of cytoplasmic lipopolysaccharides (LPS) in immune and non-immune cells. Upon detection of LPS, this inflammasome activates the signaling proteases caspase-4 and -5 (in humans) and caspase-11 (in mice). Inflammatory caspases activation leads to caspase self-processing and the cleavage of the pore-forming protein Gasdermin D (GSDMD). GSDMD N-terminal fragments oligomerize and form pores at the plasma membranes, leading to an inflammatory form of cell death called pyroptosis. Here, we describe a simple method to activate the non-canonical inflammasome in myeloid and epithelial cells and to measure its activity using cell death assay and immunoblotting.