Effects of increased venous pressure on albumin- and IgG-excluded volumes in muscle.
Effects of increased venous pressure on albumin- and IgG-excluded volumes in muscle.
复制标题
静脉压升高对肌肉中白蛋白和 IgG 排除体积的影响。
DOI：
10.1152/ajpheart.1982.242.6.h1044
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Mullins,RJ
中科院分区：
文献类型：
--
作者：
Bell,DR;Mullins,RJ
在麻醉家兔上，研究静脉压升高对肌肉中白蛋白和γ-免疫球蛋白G（IgG）血管外分布空间的影响。在9只兔子中，一条腿的静脉压从8毫米汞柱增加到28毫米汞柱，而另一条腿作为对照。在腓肠肌取样前4 h采集血浆和结前肌淋巴液样品。在结扎腘传出淋巴管后，从股淋巴管收集淋巴液。分别用51 Cr-乙二胺四乙酸（EDTA）和125 I-人血清白蛋白测定组织样品中的细胞外和血浆空间。使用火箭电免疫测定法从内源性白蛋白和IgG浓度的测量值计算蛋白质空间。在淋巴浓度下，白蛋白和IgG均从50%的间质间隙中排除。静脉充血后，间质体积是对照组的两倍。淋巴流量从0.82 +/- 0.18增加到4.44 +/- 0.64微升/分钟。白蛋白和IgG的血管外质量增加。白蛋白的排除体积分数没有变化，但IgG的排除体积分数降低。压力升高后骨骼肌间质蛋白排斥的变化可能对水肿的持续缓冲并不重要。
The effect of elevated venous pressure on the extravascular distribution space of albumin and gamma-immunoglobulin G (IgG) in muscle was studied in anesthetized rabbits. In nine rabbits, venous pressure in one leg was increased from 8 to 28 mmHg while the other leg served as a control. Samples of plasma and prenodal muscle lymph were collected for 4 h before taking samples of the gastrocnemius muscle. Lymph was collected from a femoral lymphatic after ligating the popliteal efferent lymphatic. The extracellular and plasma spaces in the tissue samples were measured using 51Cr-labeled ethylenediaminetetraacetic acid (EDTA) and 125I-human serum albumin, respectively. The protein spaces were calculated from measurements of endogenous albumin and IgG concentrations using rocket electroimmunoassay. Albumin and IgG were both excluded from 50% of the interstitial space at lymph concentration. After venous congestion, the interstitial volume was twice that of control. Lymph flow increased from 0.82 +/- 0.18 to 4.44 +/- 0.64 microliters/min. The extravascular mass of albumin and IgG increased. There was no change in the excluded volume fraction for albumin but a decrease in that for IgG. Changes in interstitial protein exclusion in skeletal muscle after pressure elevation may not be important in oncotic buffering of the edema.