Controlled release of heparin from poly(ε-caprolactone) electrospun fibers
Controlled release of heparin from poly(ε-caprolactone) electrospun fibers
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DOI：
10.1016/j.biomaterials.2005.10.028
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发表时间：
2006-03-01
期刊：
影响因子：
14
通讯作者：
Cool, SM
中科院分区：
文献类型：
--
作者：
Luong-Van, E;Grondahl, L;Cool, SM
肝素持续递送至移植血管的局部外膜表面已被证明可防止可导致移植物闭塞和失败的血管平滑肌细胞（VSMC）增殖。在这项研究中，肝素被纳入电纺聚（ε-己内酯）（PCL）纤维垫作为一种控制输送装置进行评估。具有光滑表面和没有珠缺陷的纤维可以从具有8% w/v PCL在7：3二氯甲烷：甲醇中的聚合物溶液中纺丝。随着肝素浓度的增加，观察到纤维直径显著减小。药物负载的评估和荧光标记肝素的成像显示肝素在整个纤维垫中均匀分布。在14天后，通过扩散控制从肝素/PCL纤维释放总共约一半的包封肝素。纤维在体外不诱导巨噬细胞的炎症反应，释放的肝素在防止培养的VSMCs的增殖是有效的。这些结果表明，静电纺丝PCL纤维是一个有前途的候选人提供肝素的血管损伤部位。(C)2005爱思唯尔有限公司保留所有权利。
Sustained delivery of heparin to the localized adventitial surface of grafted blood vessels has been shown to prevent the vascular smooth muscle cell (VSMC) proliferation that can lead to graft occlusion and failure. In this study heparin was incorporated into electrospun poly(epsilon-caprolactone) (PCL) fiber mats for assessment as a controlled delivery device. Fibers with smooth surfaces and no bead defects could be spun from polymer solutions with 8% w/v PCL in 7:3 dichloromethane: methanol. A significant decrease in fiber diameter was observed with increasing heparin concentration. Assessment of drug loading, and imaging of fluorescently labeled heparin showed homogenous distribution of heparin throughout the fiber mats. A total of approximately half of the encapsulated heparin was released by diffusional control from the heparin/PCL fibers after 14 days. The fibers did not induce an inflammatory response in macrophage cells in vitro and the released heparin was effective in preventing the proliferation of VSMCs in culture. These results suggest that electrospun PCL fibers are a promising candidate for delivery of heparin to the site of vascular injury. (C) 2005 Elsevier Ltd. All rights reserved.