Involvement of three glutamine tracts in human androgen receptor transactivation
Involvement of three glutamine tracts in human androgen receptor transactivation
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DOI：
10.1016/j.jsbmb.2009.10.003
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发表时间：
2010-01-01
影响因子：
4.1
通讯作者：
Inui, Hiroshi
中科院分区：
文献类型：
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作者：
Harada, Naoki;Mitani, Takakazu;Inui, Hiroshi
雄激素受体（AR）具有多态性聚谷氨酰胺束（polyQ），其长度与其转录活性呈负相关。在这里，我们研究了 AR N 末端结构域中的 6 个和 5 个重复谷氨酰胺束（分别为 Q6 和 Q5）是否也对 AR 反式激活有影响。在使用雄激素反应元件的两个串联重复的报告基因测定中，谷氨酰胺束的缺失显着增加了 AR 反式激活，顺序如下：野生型
The androgen receptor (AR) possesses a polymorphic polyglutamine tract (polyQ), whose length is inversely correlated with its transcriptional activity. Here, we investigated whether 6 and 5 repetitive glutamine tracts (Q6 and Q5, respectively) in the N-terminal domain of AR also have effects on AR transactivation. In a reporter gene assay using two-tandem repeats of an androgen response element, deletion of glutamine tracts significantly increased AR transactivation in the following order: wild-type