Protein Quantitation and Analysis of Purity
Protein Quantitation and Analysis of Purity
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DOI：
10.1007/978-1-60761-913-0_13
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Walls, Dermot
中科院分区：
文献类型：
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作者：
Campion, Eva M.;Loughran, Sinead T.;Walls, Dermot
蛋白质的准确定量及其纯度分析在许多科学研究领域都是必不可少的，并且是许多临床应用中的关键因素。因此，为这一目的而采用的大量不同技术并不令人惊讶。选择合适的检测方法取决于多种因素，如所需的灵敏度水平、干扰剂的存在以及蛋白质本身的组成。在这里，最常用的蛋白质测定方法的协议进行了概述，以及最近适应的定量免疫聚合酶链反应技术。
The accurate quantitation of proteins and an analysis of their purity are essential in numerous areas of scientific research, and are a critical factor in many clinical applications. The large and varied number of techniques employed for this purpose is therefore not surprising. The selection of a suitable assay is dependent on factors such as the level of sensitivity required, the presence of interfering agents, and the composition of the protein itself. Here, protocols for the most commonly used protein determination methodologies are outlined, as well as for the more recently adapted technique of quantitative immuno-polymerase chain reaction.