Phase-Retrieved Tomography enables Mesoscopic imaging of Opaque Tumor Spheroids.
Phase-Retrieved Tomography enables Mesoscopic imaging of Opaque Tumor Spheroids.
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DOI：
10.1038/s41598-017-12193-x
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发表时间：
2017-09-19
影响因子：
4.6
通讯作者：
Zacharakis G
中科院分区：
文献类型：
--
作者：
Ancora D;Di Battista D;Giasafaki G;Psycharakis SE;Liapis E;Ripoll J;Zacharakis G
我们提出了一种新的相位反演层析成像（PRT）方法，从根本上提高介观成像的制度超过一个传输平均自由程，并实现高分辨率，均匀地在整个体积的不透明样品。该方法利用多视图采集的混合选择性平面照明显微镜（SPIM）和光学投影断层扫描（OPT）设置和三维Gerchberg-Saxton相位检索算法应用于3D通过自相关正弦图。我们已经成功地应用这种创新的协议，以肿瘤球状体的形式对光学致密的3D细胞培养物进行成像，这是研究癌症行为和对化疗反应的高度通用模型。因此，我们已经实现了一个显着的提高分辨率的深度尚未访问与目前使用的方法在SPIM/OPT，同时克服了所有的注册和对齐问题固有的这些技术。
We present a new Phase-Retrieved Tomography (PRT) method to radically improve mesoscopic imaging at regimes beyond one transport mean-free-path and achieve high resolution, uniformly throughout the volume of opaque samples. The method exploits multi-view acquisition in a hybrid Selective Plane Illumination Microscope (SPIM) and Optical Projection Tomography (OPT) setup and a three-dimensional Gerchberg-Saxton phase-retrieval algorithm applied in 3D through the autocorrelation sinogram. We have successfully applied this innovative protocol to image optically dense 3D cell cultures in the form of tumor spheroids, highly versatile models to study cancer behavior and response to chemotherapy. We have thus achieved a significant improvement of resolution in depths not yet accessible with the currently used methods in SPIM/OPT, while overcoming all registration and alignment problems inherent to these techniques.