Modulation of sensory-CNS-motor circuits by serotonin, octopamine, and dopamine in semi-intact Drosophila larva
Modulation of sensory-CNS-motor circuits by serotonin, octopamine, and dopamine in semi-intact Drosophila larva
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DOI：
10.1016/j.neures.2003.10.005
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发表时间：
2004-02-01
影响因子：
2.9
通讯作者：
Cooper, RL
中科院分区：
文献类型：
--
作者：
Dasari, S;Cooper, RL
我们介绍了一种原位制剂，通过刺激感觉-中枢神经系统回路来诱导运动单位的活动，使用的是果蝇的三龄幼虫。根据感觉刺激的强度、持续时间和频率，可以有选择地招募和驱动在解剖和生理功能上定义良好的离散的可识别运动单位。由于周围神经系统是两侧对称的，以协调两侧对称的节段性肌肉模式，所以能够诱导出虚构的运动形式。监测突出的腹侧纵向体壁肌肉，如M6和M12上的兴奋性突触后电位(EPSP)，可以进一步深入了解选择性运动单位如何在完整的动物体内招募。我们还介绍了神经调节剂(5-羟色胺、章鱼胺(OA)和多巴胺(DA))对感觉-运动回路中可诱导的活动模式的作用。果蝇强大的遗传操作为进一步研究模式生成的回路和细胞方面以及模式生物中回路形成和维持的发育问题开辟了许多途径。(C)2003年爱思唯尔爱尔兰有限公司和日本神经科学学会。版权所有。
We have introduced an in-situ preparation to induce motor unit activity by stimulating a sensory-CNS circuit, using the third instar larvae of Drosophila melanogaster. Discrete identifiable motor units that are well defined in anatomic and physiologic function can be recruited selectively and driven depending on the sensory stimulus intensity, duration, and frequency. Since the peripheral nervous system is bilaterally symmetric to coordinate bilateral symmetric segmental musculature patterns, fictive forms of locomotion are able to be induced. Monitoring the excitatory postsynaptic potentials (EPSP) on the prominent ventral longitudinal body wall muscles, such as m6 and m12, provides additional insight into how the selective motor units might be recruited within intact animals. We also introduce the actions of the neuromodulators (serotonin, octopamine (OA) and dopamine (DA)) on the inducible patterns of activity within the sensory-motor circuit. The powerful genetic manipulation in Drosophila opens many avenues for further investigations into the circuitry and cellular aspects of pattern generation and developmental issues of circuitry formation and maintenance in the model organism. (C) 2003 Elsevier Ireland Ltd and The Japan Neuroscience Society. All rights reserved.