Proteins specified by avian erythroblastosis virus: coding region localization and identification of a previously undetected erb-B polypeptide.
Proteins specified by avian erythroblastosis virus: coding region localization and identification of a previously undetected erb-B polypeptide.
复制标题
禽成红细胞增多症病毒特异的蛋白质：编码区定位和先前未检测到的 erb-B 多肽的鉴定。
DOI：
10.1073/pnas.79.13.3958
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发表时间：
1982
影响因子：
11.1
通讯作者：
Bishop,JM
中科院分区：
文献类型：
--
作者：
Privalsky,ML;Bishop,JM
禽成红细胞病病毒（AEV）可诱导鸡成红细胞病和肉瘤。病毒基因组中的两个结构域erb-A和erb-B与aev介导的肿瘤发生有关。通过对mRNA的杂交阻滞翻译和杂交选择，我们在病毒基因组上绘制了erb结构域指定的多肽。与代表AEV mRNA剪接的5'先导区DNA杂交的结果表明，erb-B结构域的真正产物是61,000分子量的蛋白质，而不是先前鉴定的41,000分子量的多肽。
Avian erythroblastosis virus (AEV) induces erythroblastosis and sarcomas in chickens. Two domains within the viral genome, erb-A and erb-B, have been implicated in AEV-mediated oncogenesis. By use of hybridization-arrested translations and hybridization-selections of mRNA, we have mapped on the viral genome the polypeptides specified by the erb domains. The results of hybridization-arrest with DNA representing the spliced 5' leader region of the AEV mRNA suggested that the authentic product of the erb-B domain was a 61,000 molecular weight protein, not a 41,000 molecular weight polypeptide previously identified.