Synchronous fluorescence spectroscopic, immunoaffinity chromatographic and 32P-postlabeling analysis of human placental DNA known to contain benzo[a]pyrene diol epoxide adducts.
Synchronous fluorescence spectroscopic, immunoaffinity chromatographic and 32P-postlabeling analysis of human placental DNA known to contain benzo[a]pyrene diol epoxide adducts.
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对已知含有苯并[a]芘二醇环氧化物加合物的人胎盘 DNA 进行同步荧光光谱、免疫亲和色谱和 32P 标记后分析。
DOI：
10.1093/carcin/11.4.553
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发表时间：
1990
期刊：
影响因子：
4.7
通讯作者：
Harris,CC
中科院分区：
文献类型：
--
作者：
Manchester,DK;Wilson,VL;Hsu,IC;Choi,JS;Parker,NB;Mann,DL;Weston,A;Harris,CC
人类胎盘容易催化多环芳烃（PAHs）和其他致癌物质的生物转化为反应性代谢产物，这些代谢产物可以通过形成共价加合物来损伤DNA。胎盘可广泛用于流行病学研究，并可能是一个有用的剂量计致癌物暴露在人类。然而，以前的人类胎盘DNA的研究已经产生了不一致的结果与PAH-DNA加合物。为了解决围绕这些差异的一些问题，还通过32 P-后标记和免疫层析分析了已知含有苯并[a]芘二醇环氧加合物的胎盘DNA样品。结果表明，以前的差异可以解释的方法学因素影响的specificities加合物测定生物样品中，并表明，人类胎盘DNA含有来自多环芳烃的加合物。
Human placenta readily catalyzes the biotransfonnation of polycyclic aromatic hydrocarbons (PAHs) and other carcinogens to reactive metabolites that can damage DNA through formation of covalent adducts. Placenta is widely available for epidemiologic studies and may be a useful dosimeter for carcinogen exposures In humans. However, previous studies of human placental DNA have yielded discrepant results with respect to PAH—DNA adducts. In order to resolve some of the issues surrounding these discrepancies, placental DNA samples known to contain benzo[a]pyrene diol epoxide adducts were also analyzed by32P-pastlabeling and immunmnity chromatography. Results indicate that previous discrepancies can be accounted for by methodologic factors affecting the spcificities of adduct assays in biological samples and suggest that human placental DNA contains adducts derived from multiple PAHs.