Interventional Optical Imaging Permits Instant Visualization of Pathological Zones of Ablated Tumor Periphery and Residual Tumor Detection.
Interventional Optical Imaging Permits Instant Visualization of Pathological Zones of Ablated Tumor Periphery and Residual Tumor Detection.
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DOI：
10.1158/0008-5472.can-21-1040
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发表时间：
2021-09-01
期刊：
影响因子：
11.2
通讯作者：
Yang X
中科院分区：
文献类型：
--
作者：
Kan X;Zhou G;Zhang F;Ji H;Zheng H;Chick JFB;Valji K;Zheng C;Yang X
光学成像（OI）提供实时临床成像能力，同时提供疾病过程的分子、形态和功能信息。在这项研究中，我们提出了一种新的成骨不全介入技术，该技术能够在射频消融（RFA）过程中对消融肿瘤周围的三个不同病理区域进行体内可视化，以便立即检测残余肿瘤。将原位肝肿瘤兔分为不完全RFA组和完全RFA组，每组8只。以吲吲青绿（ICG）为基础的介入性成骨不全用于区分三个病理区域——消融肿瘤、过渡边缘、残余肿瘤或周围正常肝脏——并定量比较三个区域之间以及不完全消融和完全消融肿瘤之间的信号背景比（SBR）。随后进行离体成骨不全和病理对照，以证实介入成骨不全的发现。介入性成骨不全可以区分不完全或完全消融的肿瘤周围，从而可以识别残余肿瘤。这项技术可能为在单次介入消融过程中立即评估肿瘤根除开辟了新的途径。
Optical imaging (OI) provides real-time clinical imaging capability and simultaneous molecular, morphological, and functional information of disease processes. In this study, we present a new interventional OI technique which enables in vivo visualization of three distinct pathological zones of ablated tumor periphery for immediate detection of residual tumors during a radiofrequency ablation (RFA) session. Rabbits with orthotopic hepatic tumors were divided into two groups (n=8/group): incomplete RFA and complete RFA. Indocyanine green (ICG)-based interventional OI was used to differentiate three pathological zones - ablated tumor, transition margin, and residual tumor or surrounding normal liver - with quantitative comparison of signal-to-background ratios (SBR) among the three zones and between incompletely and completely ablated tumors. Subsequent ex-vivo OI and pathologic correlation were performed to confirm the findings of interventional OI. Interventional OI could differentiate incompletely or completely ablated tumor peripheries, thus permitting identification of residual tumor. This technique may open new avenues for immediate assessment of tumor eradication during a single interventional ablation session.