Tubulin genes from AIDS-associated microsporidia and implications for phylogeny and benzimidazole sensitivity.
Tubulin genes from AIDS-associated microsporidia and implications for phylogeny and benzimidazole sensitivity.
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艾滋病相关微孢子虫的微管蛋白基因及其对系统发育和苯并咪唑敏感性的影响。
DOI：
10.1016/s0166-6851(96)02628-x
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发表时间：
1996
影响因子：
1.5
通讯作者：
Edlind,TD
中科院分区：
文献类型：
--
作者：
Li,J;Katiyar,SK;Hamelin,A;Visvesvara,GS;Edlind,TD
本文报道了Hellem脑孢子虫β-微管蛋白基因的全序列（包括侧翼区），以及Septata mesinalis和Nosema locustae的部分序列。部分N。测定了蝗虫α-微管蛋白的序列。在编码108-253位残基的区域内，E. hellemandE. cuniculi，与S.与脑原虫的同源性为78%。蝗虫和脑寄生虫S.好吧与E. hellemandE. cuniculi表明β-tubulin基因为单拷贝。氨基酸序列分析表明，微孢子虫β-微管蛋白具有明显的同源性。在构巢曲霉和其它真菌中，与苯并咪唑活性有关的6个残基中，有5个存在于微孢子虫β-微管蛋白中。β-微管蛋白部分序列（108-253）的系统发育分析表明，微孢子虫与真菌粗糙脉孢菌、荚膜组织胞浆菌和卡氏肺孢子虫的序列分支较近，而与其他原生动物的序列分支较远。微孢子虫的α-微管蛋白也与真菌序列一起分支。[核苷酸序列数据提交至GenBank，登录号L47271-L47274]。
The completeEncephalitozoon hellemβ-tubulin gene sequence (including flanking regions) as well as partial sequences fromSeptata intestinalisandNosema locustaeare presented. A partialN. locustaeα-tubulin sequence was also determined. Over the region encoding residues 108-253, there was 87% identity at the nucleotide level betweenE. hellemandE. cuniculi, 85% identity betweenS. intestinalisandEncephalitozoonand 78% identity betweenN. locustaeandEncephalitozoonorS. intestinalis. Studies withE. hellemandE. cuniculiindicated that the β-tubulin gene was single copy. Amino acid sequences showed that the microsporidian β-tubulins were clearly related. 5 of 6 residues important for benzimidazole activity inAspergillus nidulansand other fungi were present in microsporidial β-tubulin. Phylogenetic analysis of partial β-tubulin sequences (residues 108-253) showed that the microsporidial sequences branched closer to those from the fungiNeurospora crassa, Histoplasma capsulatumandPneumocystis cariniithan to other protozoa. Microsporidial α-tubulin also branched with the fungal sequences. [Nucleotide sequence data submitted to GenBank, accession numbers L47271-L47274].