Identification and 3D gene expression patterns of WUSCEHEL-related homeobox (WOX) genes from Panax ginseng
Identification and 3D gene expression patterns of WUSCEHEL-related homeobox (WOX) genes from Panax ginseng
复制标题
人参 WUSCEHEL 相关同源盒 (WOX) 基因的鉴定和 3D 基因表达模式
DOI：
10.1016/j.plaphy.2019.08.027
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发表时间：
2019
影响因子：
6.5
通讯作者：
Huang Luqi
中科院分区：
文献类型：
--
作者：
Liu Juan;Jiang Chao;Chen Tong;Zha Liangping;Zhang Jie;Huang Luqi
野生人参（Panax ginseng）可以在其自然栖息地存活数百年，反映出显著的可塑性。植物干细胞（SCs）在这些植物的再生能力和终身活动中起着关键作用。WUSCHEL相关的HOMEOBOX（WOX）基因是植物SC多能性的主要调节因子，但其在药用植物中的功能尚未报道。为了研究这些基因是否定义了人参中不同的SC生态位，我们克隆并分析了人参中的五个WOXs基因（PgWOXs），发现它们可能调控根重建。然后，利用整装RNA原位杂交技术对PgWOX基因在人参幼苗和不定根中的三维表达模式进行了研究，结果表明：PgWOX 4在维管形成层SC中表达，PgWOX 5和PgWOX 11主要在幼苗和不定根的顶端表达，这两个部位是分生组织的能量中心; PgWOX 13 a和PgWOX 13 b在幼苗主根和培养的不定根的薄壁细胞中检测到，表明它们对于维持韧皮部和木质部中SC分化和自我更新之间的平衡是重要的。这是第一份关于草药SC监管的报告;我们预计P.人参可作为研究植物SC与其形态特征关系的模式药材，为通过调控植物SC提高药材产量和质量提供了新的研究方向。
Wild ginseng (Panax ginseng) can survive in their natural habitat for hundreds of years, reflecting a remarkable plasticity. Plant stem cells (SCs) play a key role in the regenerative capacity and lifelong activity of these plants. WUSCHEL-RELATED HOMEOBOX (WOX) genes are master regulators of plant SC pluripotency, but their functions in medicinal plants have not been previously reported. To investigate whether these genes define different SC niches in ginseng, we cloned and analysed fiveWOXgenes in ginseng (PgWOXs) and found that they might regulate root reconstruction. Then, the whole-mount RNAin situhybridization was used to characterize the 3D gene expression pattern ofPgWOXsin ginseng seedlings and cultured adventitious roots.PgWOX4was expressed in vascular cambium SCs;PgWOX5andPgWOX11were mainly expressed in the tips of seedling and adventitious roots, which are the energetic centre of the meristem; andPgWOX13aandPgWOX13bwere detected in the parenchyma cells of the main root of seedlings and cultured adventitious roots, suggesting that they are important for maintaining the balance between SC differentiation and self-renewal in the phloem and xylem. This is the first report of SC regulation in medical herbs; we expect thatP. ginsengcan serve as a model herb for investigating the relationship between SCs and their herbal morphological features, which would be a new research direction to improve the yield and quality of the medicinal materials by regulating the herbal SCs.