Biotin-deficient diet induces chromosome misalignment and spindle defects in mouse oocytes.
Biotin-deficient diet induces chromosome misalignment and spindle defects in mouse oocytes.
复制标题
缺乏生物素的饮食会导致小鼠卵母细胞染色体错位和纺锤体缺陷。
DOI：
10.1080/09168451.2014.968090
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Shibata K.
中科院分区：
文献类型：
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作者：
Tsuji A;Nakamura T;Shibata K.
由于减数分裂染色体错位和纺锤体缺陷导致的异常卵母细胞增加，导致不孕、流产和三体受孕的发生率升高。本文研究了生物素缺乏对卵母细胞质量的影响。3周龄雌性ICR小鼠分别饲喂生物素缺乏饲粮和对照饲粮（0.004 g /kg） 21 d。第22天，用免疫荧光法分析小鼠卵母细胞。由于生物素，营养不良增加了异常卵母细胞的频率（生物素缺乏与对照组：40%对16%）。接下来，从第22天到第42天，生物素缺乏组的其余小鼠被喂食对照或缺乏生物素的饮食。虽然恢复组的生物素营养状况有所恢复，但恢复组异常卵母细胞的出现频率仍高于对照组（48比18%）。我们的研究结果表明，稳定、充足的生物素摄入是产生高质量卵母细胞的必要条件。
Increased abnormal oocytes due to meiotic chromosome misalignment and spindle defects lead to elevated rates of infertility, miscarriage, and trisomic conceptions. Here, we investigated the effect of biotin deficiency on oocyte quality. Three-week-old female ICR mice were fed a biotin-deficient or control diet (0, 0.004 g biotin/kg diet) for 21 days. On day 22, these mouse oocytes were analyzed by immunofluorescence. Due to biotin, undernutrition increased the frequency of abnormal oocytes (the biotin deficient vs. control: 40 vs. 16%). Next, the remaining mice in the biotin-deficient group were fed a control or biotin-deficient diet from day 22 to 42. Although biotin nutritional status in the recovery group was restored, the frequency of abnormal oocytes in the recovery group was still higher than that in the control group (48 vs. 18%). Our results indicate that steady, sufficient biotin intake is required for the production of high-quality oocytes in mice.