Elastin Fiber Accumulation in Liver Correlates with the Development of Hepatocellular Carcinoma.
Elastin Fiber Accumulation in Liver Correlates with the Development of Hepatocellular Carcinoma.
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DOI：
10.1371/journal.pone.0154558
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Izumi N
中科院分区：
文献类型：
--
作者：
Yasui Y;Abe T;Kurosaki M;Higuchi M;Komiyama Y;Yoshida T;Hayashi T;Kuwabara K;Takaura K;Nakakuki N;Takada H;Tamaki N;Suzuki S;Nakanishi H;Tsuchiya K;Itakura J;Takahashi Y;Hashiguchi A;Sakamoto M;Izumi N
通过胶原纤维的分布模式评估的纤维化阶段是丙型肝炎患者发生肝细胞癌（HCC）的主要预测因子。同时，弹性蛋白纤维的作用尚未阐明。本研究的目的是确定量化胶原蛋白和弹性蛋白纤维的重要性。我们连续招募了 189 名患有丙型肝炎和晚期纤维化的患者。使用 Elastica van Gieson 染色的预处理肝活检的全切片图像，使用自动计算方法逐像素（0.46 μm/像素）评估胶原蛋白和弹性蛋白纤维。因此，分析了纤维量和3年内HCC的累积发病率。胶原蛋白和弹性蛋白纤维之间存在显着相关性，而弹性蛋白纤维的变化大于胶原纤维。胶原纤维 (p = 0.008) 和弹性蛋白纤维 (p < 0.001) 均与 F 期显着相关。随访期间总共有 30 名患者发展为 HCC。具有较高弹性蛋白纤维 (p = 0.002) 和较高胶原纤维 (p = 0.05) 的患者的 HCC 发病率显着较高。至于弹性蛋白纤维，这种差异在 F3 患者中仍然显着。此外，对于胶原纤维含量较高的患者，较高的弹性蛋白是 HCC 发展的重要预测因子 (p = 0.02)。计算分析是一种定量纤维的新技术，具有传统分级的附加值。弹性蛋白纤维是 HCC 发展的预测因子，与胶原纤维和 F 期无关。
The fibrosis stage, which is evaluated by the distribution pattern of collagen fibers, is a major predictor for the development of hepatocellular carcinoma (HCC) for patients with hepatitis C. Meanwhile, the role of elastin fibers has not yet been elucidated. The present study was conducted to determine the significance of quantifying both collagen and elastin fibers. We enrolled 189 consecutive patients with hepatitis C and advanced fibrosis. Using Elastica van Gieson-stained whole-slide images of pretreatment liver biopsies, collagen and elastin fibers were evaluated pixel by pixel (0.46 μm/pixel) using an automated computational method. Consequently, fiber amount and cumulative incidences of HCC within 3 years were analyzed. There was a significant correlation between collagen and elastin fibers, whereas variation in elastin fiber was greater than in collagen fiber. Both collagen fiber (p = 0.008) and elastin fiber (p < 0.001) were significantly correlated with F stage. In total, 30 patients developed HCC during follow-up. Patients who have higher elastin fiber (p = 0.002) in addition to higher collagen fiber (p = 0.05) showed significantly higher incidences of HCC. With regard to elastin fiber, this difference remained significant in F3 patients. Furthermore, for patients with a higher collagen fiber amount, higher elastin was a significant predictor for HCC development (p = 0.02). Computational analysis is a novel technique for quantification of fibers with the added value of conventional staging. Elastin fiber is a predictor for the development of HCC independently of collagen fiber and F stage.