A comparative study of triacylglycerol composition in Chinese human milk within different lactation stages and imported infant formula by SFC coupled with Q-TOF-MS
A comparative study of triacylglycerol composition in Chinese human milk within different lactation stages and imported infant formula by SFC coupled with Q-TOF-MS
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DOI：
10.1016/j.foodchem.2016.11.139
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发表时间：
2017-04-15
期刊：
影响因子：
8.8
通讯作者：
Du, Zhenxia
中科院分区：
文献类型：
--
作者：
Tu, Anqi;Ma, Qiang;Du, Zhenxia
三酰甘油（TAGs）作为乳脂的主要成分，是保证婴儿健康成长的重要因素。使用超临界流体色谱 (SFC) 与四极飞行时间质谱 (Q-TOF-MS) 相结合，建立了一种识别母乳 (HM) 和婴儿配方奶粉 (IF) 中 TAG 的有效方法。结果表明，该方法具有良好的回收率（>80%）和相关系数（r(2) >= 0.993）的可行性。 HM 中鉴定出 60 多个 TAG，IF 中鉴定出 50 多个 TAG。分析结果表明，HM 中的 TAG 受哺乳期影响很大。 HM和IF之间存在显着差异，例如IF中的中链TAG和饱和TAG要高得多，这表明国外制造商开发的配方奶粉不适合中国婴儿。这种高通量方法在牛奶样品分析方面展现出巨大的潜力，其结果可能为中国婴儿饮食提供重要指导。 (C) 2016 Elsevier Ltd. 保留所有权利。
Triacylglycerols (TAGs) as the major component of milk fat are significant factors to ensure the healthy growth of infants. An efficient method for identifying TAGs in human milk (HM) and infant formula (IF) was established using supercritical fluid chromatograph (SFC) coupled with quadruple time-of-flight mass spectrometry (Q-TOF-MS). The results indicated the feasibility of this method with satisfactory recoveries (>80%) and correlation coefficients (r(2) >= 0.993). More than 60 TAGs in HM and 50 TAGs in IF were identified. The profiling results demonstrated that TAGs in HM were greatly affected by lactation stage. Significant differences were found between HM and IF, such as much higher medium chain TAGs and saturated TAGs in IF, indicating that the formulas developed by foreign manufacturers were not suitable for Chinese babies. This high-throughput method exhibits a huge potential for analysis of milk samples and the result may serve as an important guide for Chinese infants diet. (C) 2016 Elsevier Ltd. All rights reserved.