The Effect of Synthetic Salidroside on Cytokines and Airway Inflammation of Asthma Induced by Diisocyanate (TDI) in Mice by Regulating GATA3/T-bet
The Effect of Synthetic Salidroside on Cytokines and Airway Inflammation of Asthma Induced by Diisocyanate (TDI) in Mice by Regulating GATA3/T-bet
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DOI：
10.1007/s10753-014-9979-x
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发表时间：
2015-04-01
期刊：
影响因子：
5.1
通讯作者：
Yan, Tianhua
中科院分区：
文献类型：
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作者：
Wang, Jing;Xiao, Lu;Yan, Tianhua
本研究旨在探讨人工合成红景天苷对二异氰酸酯（TDI）诱导的哮喘小鼠细胞因子及气道炎症的影响。将60只雌性BALB/C小鼠随机分为6个实验组：对照组、赋形剂组、模型组、地塞米松（2 mg/kg）组和红景天苷（24和48 mg/kg）组。实验结束后，采用苏木精-伊红染色法进行组织学观察，并采集动物支气管肺泡灌洗液（BALF）和血液，检测诱导的炎症细胞组成和某些细胞因子（IL-4、INF-γ）的浓度。通过QPCR评估GATA 3和T-bet mRNA。我们的研究表明，红景天苷抑制TDI诱导的嗜酸性粒细胞计数增加; IL-4和INF-γ恢复。组织学研究表明，红景天苷实质上抑制TDI诱导的肺组织嗜酸性粒细胞增多。红景天苷可提高肺组织T-bet mRNA表达，降低GATA 3 mRNA表达。这些结果表明红景天苷可能通过调节GATA 3/T-bet平衡而有效地改善哮喘的进展，并可作为过敏性哮喘患者的治疗药物。
This study was conducted to explore the anti-asthma effects of synthetic salidroside on cytokines and airway inflammation of asthma induced by diisocyanate (TDI) in mice. The experiment was carried out 60 female BALB/C mice which were randomly assigned to six experimental groups: control, vehicle, model, dexamethasone (2 mg/kg), and salidroside (24 and 48 mg/kg). After the experiment, histological studies were evaluated by the hematoxylin and eosin staining, the bronchoalveolar lavage fluid (BALF) and blood were collected from the animals, and the composition of the induced inflammatory cells, and the concentrations of certain cytokines (IL-4, INF-gamma) were evaluated. GATA3 and T-bet mRNAs were evaluated by QPCR. Our study demonstrated that salidroside inhibited TDI-induced increases in eosinophil count; IL-4 and INF-gamma were recovered. Histological studies demonstrated that salidroside substantially inhibited TDI-induced eosinophilia in lung tissue. Salidroside can improve T-bet mRNA and reduce GATA3 mRNA in lung. These findings suggest that salidroside may effectively ameliorate the progression of asthma and could be used as a therapy for patients with allergic asthma by regulating GATA3/T-bet balance.