Co-immobilization of Candida rugosa and Rhyzopus oryzae lipases and biodiesel production
Co-immobilization of Candida rugosa and Rhyzopus oryzae lipases and biodiesel production
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DOI：
10.1007/s11814-013-0058-z
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发表时间：
2013-06-01
影响因子：
2.7
通讯作者：
Kim, Seung Wook
中科院分区：
文献类型：
--
作者：
Lee, Jong Ho;Kim, Sung Bong;Kim, Seung Wook
采用硅胶为固定化载体，酶前处理后同时固定化皱纹假丝酵母脂肪酶和米里佐霉脂肪酶。考察了反应时间、酶比等因素对共固定化过程的影响。然后用共固定化的酶基质生产生物柴油。采用分批进料甲醇的间歇反应系统，采用固定床反应器连续进料。在最佳固定化条件下，酶活约为16,000 U/g中心点阵。当共固定化酶与优化的分步进料甲醇配合使用时，生物柴油的转化率在3h可达99%左右，并可重复使用约30次，转化率仍保持在90%以上。
Candida rugosa lipase and Ryzopus oryzae lipase were simultaneously immobilized on silica gel following enzyme pretreatment. The factors affecting the co-immobilization process, such as reaction time and enzyme ratio, were investigated. Biodiesel was then produced by using the co-immobilized enzyme matrix. A batch system was employed with stepwise methanol feeding, and the continuous process involved a packed-bed reactor. Under optimal immobilization conditions, the activity was approximately 16,000 U/g center dot matrix. When co-immobilized enzyme was used with optimized stepwise methanol feeding, conversion of biodiesel reached about 99% at 3 h and was maintained at a level of over 90% for about 30 reuses.