Adiponectin gene expression and adipocyte diameter: a comparison between epicardial and subcutaneous adipose tissue in men.
Adiponectin gene expression and adipocyte diameter: a comparison between epicardial and subcutaneous adipose tissue in men.
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DOI：
10.1016/j.carpath.2010.07.005
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发表时间：
2011-09-01
期刊：
影响因子：
--
通讯作者：
Zamboni, Mauro
中科院分区：
文献类型：
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作者：
Bambace, Clara;Telesca, Mariassunta;Zamboni, Mauro
简介：最近的兴趣集中在心外膜脂肪，但鲜为人知的是心外膜脂肪细胞的大小及其与胰岛素抵抗和adipokines.METHODS的关系：从皮下，心外膜，和腹膜脂肪从21名男性进行择期心脏手术，无论是冠状动脉旁路移植术（n=11）或瓣膜置换术（n=10）收集活检。我们评估了心外膜脂肪细胞的大小，并将其与皮下脂肪和腹膜脂肪进行比较。通过使用胶原酶消化脂肪组织、通过离心分离脂肪细胞、亚甲蓝染色细胞核和测量细胞直径来确定脂肪细胞大小。测定患者的体重、身高、体重指数、腰围，以及血糖、胰岛素、稳态模型评估指数、脂联素、瘦素血清水平。脂联素mRNA的水平通过实时聚合酶链反应测定皮下脂肪和心外膜脂肪活检。结果：心外膜脂肪中的脂肪细胞显着小于皮下脂肪和腹膜脂肪。心外膜脂肪细胞大小与胰岛素抵抗、血清瘦素呈正相关，与血清脂联素及脂联素mRNA表达呈负相关。心外膜脂肪中脂联素mRNA的表达明显低于皮下脂肪。心脏瓣膜置换术患者的心外膜脂肪中脂肪细胞的大小明显小于冠状动脉旁路移植术患者。脂联素基因表达在后者低于前者，虽然没有显着。结论：脂肪细胞在心外膜脂肪小于腹膜和皮下脂肪。心外膜和皮下脂肪中的脂肪细胞大小与胰岛素抵抗呈正相关，与局部脂联素基因表达呈负相关，并且在冠状动脉疾病受试者中减少。心外膜中的脂联素基因表达明显低于皮下脂肪。
INTRODUCTION: Interest has recently focused on epicardial fat, but little is known about epicardial adipocyte size and its relation with insulin resistance and adipokines.METHODS: Biopsies were collected from subcutaneous, epicardial-, and peritoneal fat from 21 males undergoing elective cardiac surgery either for coronary artery bypass grafting (n=11) or for valve replacement (n=10). We assessed epicardial adipocyte size, comparing it with that from subcutaneous fat and peritoneal fat. The adipocyte size was determined by using collagenase digestion of adipose tissue, separation of adipocytes by centrifugation, methylene blue staining of the nuclei, and measurement of the cell diameter. Patient's weight, height, body mass index, waist, as well as glucose, insulin, homeostatic model assessment index, adiponectin, and leptin serum levels were determined. Adiponectin mRNA levels were determined by real-time polymerase chain reaction on subcutaneous fat and epicardial fat biopsies.RESULTS: Adipocytes in epicardial fat were significantly smaller than those in subcutaneous and peritoneal fat. The adipocyte size in epicardial fat correlated positively with insulin resistance and serum leptin, and correlated negatively with serum and mRNA expression of adiponectin. Adiponectin mRNA expression in epicardial fat was significantly lower than in subcutaneous fat. Adipocyte size in epicardial fat was significantly smaller in valve-replacement patients than in coronary artery bypass graft patients. Adiponectin gene expression was lower in the latter than in the former, although not significantly.CONCLUSIONS: Adipocytes in epicardial fat are smaller than those in peritoneal and subcutaneous fat. Adipocyte size, both in epicardial and in subcutaneous fat, is positively related with insulin resistance, shows negative association with local adiponectin gene expression, and is decreased in subjects with coronary artery disease. Adiponectin gene expression is significantly lower in epicardial- than in subcutaneous fat.