On‐tissue chemical derivatization of volatile metabolites for matrix‐assisted laser desorption/ionization mass spectrometry imaging
On‐tissue chemical derivatization of volatile metabolites for matrix‐assisted laser desorption/ionization mass spectrometry imaging
复制标题
用于基质辅助激光解吸/电离质谱成像的挥发性代谢物的组织化学衍生化
DOI：
10.1002/jms.4918
复制
发表时间：
2023
影响因子：
2.3
通讯作者：
Lee, Young Jin
中科院分区：
文献类型：
--
作者：
Forsman, Trevor T.;Paulson, Andrew E.;Larson, Evan A.;Looft, Torey;Lee, Young Jin
挥发性代谢物的质谱成像（MSI）具有挑战性，特别是在基质辅助激光解吸/电离（MALDI）中。大多数MALDI离子源在真空中工作，这导致分析过程中挥发性代谢物的蒸发。此外，组织样品在样品制备过程中经常被干燥，导致挥发性代谢物的损失，即使对于其他MSI技术也是如此。组织上化学衍生可以显著降低分析物的挥发性。在此，提出了一种衍生化方法，在制备用于MSI可视化的样品之前，利用N，N，N-三甲基-2-（哌嗪-1-基）乙烷-1-碘化铵对鸡盲肠、回肠和空肠组织切片中的短链脂肪酸进行化学修饰。
Mass spectrometry imaging (MSI) of volatile metabolites is challenging, especially in matrix‐assisted laser desorption/ionization (MALDI). Most MALDI ion sources operate in vacuum, which leads to the vaporization of volatile metabolites during analysis. In addition, tissue samples are often dried during sample preparation, leading to the loss of volatile metabolites even for other MSI techniques. On‐tissue chemical derivatization can dramatically reduce the volatility of analytes. Herein, a derivatization method is proposed utilizing N,N,N‐trimethyl‐2‐(piperazin‐1‐yl)ethan‐1‐aminium iodide to chemically modify short‐chain fatty acids in chicken cecum, ileum, and jejunum tissue sections before sample preparation for MSI visualization.