Estimation of evolutionary distance between nucleotide sequences.
Estimation of evolutionary distance between nucleotide sequences.
复制标题
DOI：
10.1093/oxfordjournals.molbev.a040317
复制
发表时间：
1984-04
影响因子：
10.7
通讯作者：
F. Tajima;M. Nei
中科院分区：
文献类型：
--
作者：
F. Tajima;M. Nei
通过考虑不同核苷酸对之间不等的取代率，开发了一个数学公式，用于估计两个同源 DNA 序列之间每个位点的平均核苷酸取代数 (delta)。虽然这个公式是针对核苷酸取代的等输入模型获得的，但计算机模拟表明，只要 delta 等于或小于 1，它就可以对各种核苷酸取代模式给出相当好的估计。此外，该公式不适用的情况频率远低于最近提出的其他类似方法。这一点可以用胰岛素基因来说明。还开发了一种用于估计由于缺失和插入而引起的核苷酸变化数量的统计方法。将此方法应用于珠蛋白基因数据表明，每个位点的核苷酸变化数量随着进化时间而增加，但增加的模式非常不规则。
A mathematical formula for estimating the average number of nucleotide substitutions per site (delta) between two homologous DNA sequences is developed by taking into account unequal rates of substitution among different nucleotide pairs. Although this formula is obtained for the equal-input model of nucleotide substitution, computer simulations have shown that it gives a reasonably good estimate for a wide range of nucleotide substitution patterns as long as delta is equal to or smaller than 1. Furthermore, the frequency of cases to which the formula is inapplicable is much lower than that for other similar methods recently proposed. This point is illustrated using insulin genes. A statistical method for estimating the number of nucleotide changes due to deletion and insertion is also developed. Application of this method to globin gene data indicates that the number of nucleotide changes per site increases with evolutionary time but the pattern of the increase is quite irregular.