Threonine deficiency decreased intestinal immunity and aggravated inflammation associated with NF-κB and i signalling pathways in juvenile grass carp (Ctenopharyngodon idella) after infection with Aeromonas hydrophila
Threonine deficiency decreased intestinal immunity and aggravated inflammation associated with NF-κB and i signalling pathways in juvenile grass carp (Ctenopharyngodon idella) after infection with Aeromonas hydrophila
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苏氨酸缺乏会降低草鱼幼鱼（Ctenopharyngodon idella）感染嗜水气单胞菌后的肠道免疫力，并加重与 NF-κ B 和 i 信号通路相关的炎症
DOI：
10.1017/s0007114517001830
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发表时间：
2017-07-01
影响因子：
3.6
通讯作者：
Feng, Lin
中科院分区：
文献类型：
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作者：
Dong, Yu-Wen;Jiang, Wei-Dan;Feng, Lin
本研究旨在探讨饲料中添加苏氨酸对草鱼幼鱼肠道免疫功能和炎症反应的影响。配制了6种苏氨酸水平(3.99~21.66g苏氨酸/kg)的等氮半纯饲料，饲养8周，然后用嗜水气单胞菌攻击14d。结果表明，与最佳苏氨酸水平相比，苏氨酸缺乏(1)降低了鱼类的抗肠炎能力、肠道溶菌酶活性(除远端肠道外)、酸性磷酸酶活性、补体3(C3)、C4含量和IgM含量(除近端肠道(PI)外)，并下调了肝脏表达的抗菌肽(LEAP)-2A、-2B、HepLEAP-2A、CIDIN、IGZ、IgM的转录丰度免疫球蛋白和β-防御素1(PI除外)(P<；(2)可上调肠道促炎细胞因子TNF-α、IL-1β、IL-6、IL-8和IL-17D的mRNA水平，部分与核因子-kappaB信号有关；(3)可下调肠道抗炎细胞因子转化生长因子-β(1)、转化生长因子-β(2)、IL-4/13A(不是IL-4/13B)和IL-10mRNA的表达，部分是通过雷帕霉素信号转导的靶点。根据特定生长率、肠炎发病率和免疫球蛋白含量，苏氨酸的适宜需要量分别为14.53g苏氨酸/kg饲料(4.48g苏氨酸/100g蛋白质)、15.05g苏氨酸/kg饲料(4.64g苏氨酸/100g蛋白质)和15.17g苏氨酸/kg饲料(4.68g苏氨酸/100g蛋白质)。
This study aimed to investigate the impacts of dietary threonine on intestinal immunity and inflammation in juvenile grass carp. Six isonitrogenous semi-purified diets containing graded levels of threonine (3.99-21.66 g threonine/kg) were formulated and fed to fishes for 8 weeks, and then challenged with Aeromonas hydrophila for 14 d. Results showed that, compared with optimum threonine supplementation, threonine deficiency (1) decreased the ability of fish against enteritis, intestinal lysozyme activities (except in the distal intestine), acid phosphatase activities, complement 3 (C3) and C4 contents and IgM contents (except in the proximal intestine (PI)), and it down-regulated the transcript abundances of liver-expressed antimicrobial peptide (LEAP)-2A, LEAP-2B, hepcidin, IgZ, IgM and beta-defensin1 (except in the PI) (P < 0.05); (2) could up-regulate intestinal pro-inflammatory cytokines TNF-alpha, IL-1 beta, IL-6, IL-8 and IL-17D mRNA levels partly related to NF-kappa B signalling; (3) could down-regulate intestinal anti-inflammatory cytokine transforming growth factor (TGF)-beta(1), TGF-beta(2), IL-4/13A (not IL-4/13B) and IL-10 mRNA levels partly by target of rapamycin signalling. Finally, on the basis of the specific growth rate, against the enteritis morbidity and IgM contents, the optimum threonine requirements were estimated to be 14.53 g threonine/kg diet (4.48 g threonine/100 g protein), 15.05 g threonine/kg diet (4.64 g threonine/100 g protein) and 15.17 g threonine/kg diet (4.68 g threonine/100 g protein), respectively.