Flow cytometric analysis of deparaffinized nuclei in urinary bladder carcinoma. Comparison with cytogenetic analysis.
Flow cytometric analysis of deparaffinized nuclei in urinary bladder carcinoma. Comparison with cytogenetic analysis.
复制标题
膀胱癌脱蜡细胞核的流式细胞术分析。
DOI：
10.1002/1097-0142(19860415)57:8
复制
发表时间：
1986
期刊：
影响因子：
6.2
通讯作者：
Weinstein,RS
中科院分区：
文献类型：
--
作者：
Coon,JS;Schwartz,D;Summers,JL;Miller3rd,AW;Weinstein,RS
细胞遗传学分析和流式细胞术(FCM)在膀胱移行细胞癌(TCC)预后分析中均有一定的应用价值。为了对比这两种方法的结果，我们研究了一组独特的患者，他们的肿瘤已经接受了前瞻性的常规细胞遗传学分析。对这些肿瘤的石蜡切片进行流式细胞术，然后分析核DNA含量。在处理的34个肿瘤中，30个(88%)产生了可解释的DNA直方图。在其中26例(87%)中，两种方法在存在或不存在超二倍体细胞系方面具有良好的相关性。差异可能是由于采样误差或将四倍体峰解释为显著的G2M区域造成的。回顾流式细胞术分析石蜡包埋的膀胱移行细胞癌组织与传统的、前瞻性的细胞遗传学分析有很好的相关性，适用于大多数膀胱移行细胞癌。
Both cytogenetic analysis and flow cytometry (FCM) have prognostic efficacy in the analysis of transitional cell carcinoma (TCC) of urinary bladder. To correlate results of the two methods, we studied a unique group of patients whose tumors had undergone prospective conventional cytogenetic analysis. Paraffin blocks of these tumors were processed for FCM, then analyzed for nuclear DNA content. Of the 34 tumors processed, 30 (88%) yielded interpretable DNA histograms. In 26 (87%) of these, there was good correlation between the two methods with respect to the presence or absence of a hyperdiploid cell line. Discrepancies may have resulted from sampling error or from interpretation of a tetraploid peak as a prominent G2M region. Retrospective FCM analysis of paraffinized TCC tissue correlates well with conventional, prospective cytogenetic analysis and is applicable to the majority of urinary bladder transitional cell carcinomas.Cancer57:1594–1601, 1986.