Detection of SARS-CoV Antigen via SPR Analytical Systems with Reference
Detection of SARS-CoV Antigen via SPR Analytical Systems with Reference
复制标题
通过 SPR 分析系统检测 SARS-CoV 抗原并参考
DOI：
10.5772/7209
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Yujie Wang
中科院分区：
文献类型：
--
作者：
D. Cui;Xing Chen;Yujie Wang
表面等离子体共振（SPR）是一种用于检测在金属界面处发生的折射率变化的极其灵敏的光学技术，其可以在没有标记要求的情况下真实的时间监测过程（Lee等人，2006; Kanda等人，2004; Homola，2003）。自1989年以来，我们一直专注于SPR技术的开发。几代SPR分析平台，包括参考SPR分析系统，电化学SPR分析系统，便携式SPR分析系统，和高分辨率，多分析物成像SPR分析系统，已经被设计，制造和测试用于各种应用。严重急性呼吸道综合征（SARS）是一种严重的传染病，2003年在我国造成了巨大的损失。它是由SARS相关冠状病毒（SARS-CoV）感染引起的（Rota et al，2003），SARS的早期诊断对于更好地控制未来的SARS流行非常重要（Drosten et al，2003）。目前医院使用的诊断方法有多种，但没有一种方法可以用于低成本的早期发现。例如，ELISA无法检测感染患者在感染的前两周内产生的抗体。本章利用自行研制的参考型SPR仪器，在单个流动池中对反应点进行监控，建立了一种新的SARS-CoV抗原检测方法。SARS-CoV单克隆抗体通过选择性化学修饰仅固定在反应点上，而不固定在参考点上。在此基础上，研制了一种高通量、多分析物成像SPR（HMI-SPR）分析系统，并成功地进行了测试
Surface Plasmon Resonance (SPR) is an extremely sensitive optical technique to detect the changes in refractive index occurring at the metal interface, which can monitor the processes in real time without labelling requirements (Lee et al., 2006; Kanda et al., 2004; Homola, 2003). Since 1989, our group have been focusing on SPR technology development. Several generations of SPR analytical platforms, including Reference SPR Analysis System, Electrochemical-SPR Analysis System, Portable SPR Analysis System, and HighThroughput, Multi-analyte Imaging SPR Analysis System, have been designed, fabricated and tested for various applications. SARS (Sever Acute Respiratory Syndrome) is a kind of serious infection, which caused tremendous loss in China at 2003. It is caused by the infection with SARS-associated coronavirus (SARS-CoV) (Rota et al, 2003) and early diagnosis of SARS is very important for better control of future SARS epidemics (Drosten et al, 2003). There have been several diagnostic methods currently used in hospital, but none of them can be used for early detection with low cost. For example, ELISA cannot detect antibodies that were produced in the infected patients within the first two-week’s infections.. In this chapter, a novel detection method of the SARS-CoV antigens was reported by using a home-developed Reference SPR Instrument, which can monitor reaction spots with control in a single flow cell. Monoclonal antibodies to SARS-CoV were immobilized only on the reaction spots via selective chemical modification, with no immobilization on the reference spots. Then a higher version of reference SPR analytical system: high-throughput, multianalyte imaging SPR (HMI-SPR) analytical system, was developed and tested successfully