CARBOHYDRATE METABOLISM AND RESPIRATION OF ISOLATED SMALL LYMPHOCYTES - IN VITRO STUDIES OF NORMAL AND PHYTOHEMAGGLUTININ STIMULATED CELLS
CARBOHYDRATE METABOLISM AND RESPIRATION OF ISOLATED SMALL LYMPHOCYTES - IN VITRO STUDIES OF NORMAL AND PHYTOHEMAGGLUTININ STIMULATED CELLS
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DOI：
10.1182/blood.v30.6.691.691
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发表时间：
1967-01-01
期刊：
影响因子：
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通讯作者：
PACHMAN, LM
中科院分区：
文献类型：
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作者：
PACHMAN, LM
从马血中分离出平均大小为6.7微米的均一小淋巴细胞群，并在体外保持基本完全存活24小时。存活5天，存活率为50%。小淋巴细胞消耗葡萄糖的速率为1.87Mumoles/1×107 cell/min，产生乳酸的速率为2.30Mumoles/1×107cell/min。用Clark氧电极测得马小淋巴细胞的耗氧量为1.023[正负]摩尔氧气/分钟；混合外周血白细胞的氧耗量为1.25[正负]摩尔氧气/1×107细胞/分钟。淋巴细胞糖酵解在厌氧条件下被刺激(巴斯德效应)，24小时后活力不受影响。在氮气环境中。通过加入2，4-二硝基苯酚来解偶联氧化磷酸化，可以刺激呼吸和糖酵解。正常小马淋巴细胞的糖原含量为7.45[正负]1.04微克葡萄糖当量/1×107细胞。在组织培养液中加入植物血胶后，许多最初的细胞群转变为“母细胞”。这种反应与糖酵解和呼吸速率增加24小时有关。细胞内糖原上升48小时。
A homogeneous population of small lymphocytes with ar average size of 6.7 microns was isolated from equine blood and maintained in vitro with essentially complete survival for 24 hr. with a 50% viability for 5 days. The small lymphocytes consumed glucose at a rate of 1.87 m umoles/1 X 107 cells/minute, and produced lactic acid at a rate of 2.30 m umoles/1 X 107 cells/ minute. The O2 consumption of small equine lymphocytes was 1.023 [plus or minus] .165 m [mu]moles oxygen 1 X 107 cells/minute, and that of mixed peripheral blood leukocytes, 1.25 [plus or minus] .07 m [mu]moles oxygen/1 X 107 cells/ minute, as determined using a Clark oxygen electrode. Lymphocyte glycolysis was stimulated under anaerobic conditions (Pasteur effect), and viability was unimpaired after 24 hr. in a N2 environment. Uncoupling of oxidative phosphorylation by the addition of 2,4 dinitro-phenol stimulated both respiration and glycolysis. The glycogen content of the normal small horse lymphocyte was 7.45 [plus or minus] 1.04 ug glucose equivalents per 1 X 107 cells. Many of the initial cell population were transformed into "blasts" following the addition of phytohemag-glutin to the tissue culture medium. This response was associated with an increase in the rate of glycolysis and respiration by 24 hr. and a rise in intracellular glycogen by 48 hr.