Mitochondrial 'kiss-and-run': interplay between mitochondrial motility and fusion-fission dynamics
Mitochondrial 'kiss-and-run': interplay between mitochondrial motility and fusion-fission dynamics
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DOI：
10.1038/emboj.2009.255
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发表时间：
2009-10-21
期刊：
影响因子：
11.4
通讯作者：
Hajnoczky, Gyoergy
中科院分区：
文献类型：
--
作者：
Liu, Xingguo;Weaver, David;Hajnoczky, Gyoergy
在真实的时间内可视化线粒体融合，我们确定了哺乳动物细胞中的两类融合事件。除了完全融合，我们观察到短暂的融合事件，其中两个线粒体进入紧密并置，交换可溶性膜间空间和基质蛋白，并重新分离，保持原始形态。瞬时融合表现出快速的动力学的顺序和可分离的合并的外膜和内膜，但只允许部分交换的完整的膜蛋白。当内膜融合蛋白Opa 1水平降低或显著升高时，可发生短暂融合，而完全融合消失。此外，瞬时融合开始于与单独的微管相关的线粒体的倾斜或侧向相互作用，而完全融合则是由沿着沿着单个微管行进的细胞器的纵向合并引起的。与完全融合相反，瞬时融合需要并促进线粒体运动。瞬时融合也是必要的，足以支持线粒体代谢。因此，Opa 1表达和细胞骨架锚定管理一种新的融合形式，在线粒体维护中具有独特的功能。The EMBO Journal（2009）28，3074-3089. doi：10.1038/doj.2009.255; 2009年9月10日在线发布
Visualizing mitochondrial fusion in real time, we identified two classes of fusion events in mammalian cells. In addition to complete fusion, we observed transient fusion events, wherein two mitochondria came into close apposition, exchanged soluble inter-membrane space and matrix proteins, and re-separated, preserving the original morphology. Transient fusion exhibited rapid kinetics of the sequential and separable mergers of the outer and inner membranes, but allowed only partial exchange of integral membrane proteins. When the inner membrane fusion protein Opa1 level was lowered or was greatly elevated, transient fusions could occur, whereas complete fusions disappeared. Furthermore, transient fusions began from oblique or lateral interactions of mitochondria associated with separate microtubules, whereas complete fusions resulted from longitudinal merging of organelles travelling along a single microtubule. In contrast to complete fusion, transient fusions both required and promoted mitochondrial motility. Transient fusions were also necessary and sufficient to support mitochondrial metabolism. Thus, Opa1 expression and cytoskeletal anchorage govern a novel form of fusion that has a distinct function in mitochondrial maintenance. The EMBO Journal (2009) 28, 3074-3089. doi:10.1038/emboj.2009.255; Published online 10 September 2009