Myotubularin phosphoinositide phosphatases, protein phosphatases, and kinases: their roles in junction dynamics and spermatogenesis.
Myotubularin phosphoinositide phosphatases, protein phosphatases, and kinases: their roles in junction dynamics and spermatogenesis.
复制标题
肌管蛋白磷酸肌醇磷酸酶、蛋白磷酸酶和激酶：它们在连接动力学和精子发生中的作用。
DOI：
10.1002/jcp.20303
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发表时间：
2005
影响因子：
5.6
通讯作者：
Cheng,CYan
中科院分区：
文献类型：
--
作者：
Zhang,Jiayi;Mruk,DoloresD;Cheng,CYan
哺乳动物睾丸生精上皮的精子发生是一个动态的细胞事件。它涉及支持-生殖细胞界面的广泛重组，允许生殖细胞从基底到近腔室穿过上皮。因此，支持-生殖细胞肌动蛋白基粘附连接（AJ），如外质特化（ES），必须拆卸和重新组装，以促进这一事件。最近的研究表明，AJ动力学受AJ整合膜蛋白（例如，钙粘蛋白、α6β1整联蛋白和连接蛋白）、磷酸酶、激酶、衔接子和潜在的细胞骨架网络。例如，肌管蛋白（MTM）磷酸肌醇（PI）磷酸酶，如MTM相关蛋白2（MTMR 2），可以与c-Src（一种非受体蛋白酪氨酸激酶）形成功能复合物。反过来，这种磷酸酶/激酶复合物与β-连环蛋白结合，β-连环蛋白是AJ位点的N-钙粘蛋白/β-连环蛋白功能单元的组成部分。这种MTMR 2-c-Src-β-catenin复合物明显调节β-catenin的磷酸化状态，其决定生精上皮中钙粘蛋白-catenin蛋白复合物赋予的细胞粘附功能。在这篇综述中，我们讨论了目前的研究现状，选择磷酸酶和激酶，以及这些蛋白质如何潜在的相互作用与衔接子在AJ在生精上皮细胞在睾丸中调节细胞粘附。还强调了开放供进一步调查的具体研究领域。© 2005 Wiley利斯公司
Spermatogenesis in the seminiferous epithelium of the mammalian testis is a dynamic cellular event. It involves extensive restructuring at the Sertoli–germ cell interface, permitting germ cells to traverse the epithelium from basal to adluminal compartment. As such, Sertoli–germ cell actin‐based adherens junctions (AJ), such as ectoplasmic specializations (ES), must disassemble and reassemble to facilitate this event. Recent studies have shown that AJ dynamics are regulated by intricate interactions between AJ integral membrane proteins (e.g., cadherins, α6β1 integrins and nectins), phosphatases, kinases, adaptors, and the underlying cytoskeleton network. For instance, the myotubularin (MTM) phosphoinositide (PI) phosphatases, such as MTM related protein 2 (MTMR2), can form a functional complex with c‐Src (a non‐receptor protein tyrosine kinase). In turn, this phosphatase/kinase complex associates with β‐catenin, a constituent of the N‐cadherin/β‐catenin functional unit at the AJ site. This MTMR2‐c‐Src‐β‐catenin complex apparently regulates the phosphorylation status of β‐catenin, which determines cell adhesive function conferred by the cadherin–catenin protein complex in the seminiferous epithelium. In this review, we discuss the current status of research on selected phosphatases and kinases, and how these proteins potentially interact with adaptors at AJ in the seminiferous epithelium to regulate cell adhesion in the testis. Specific research areas that are open for further investigation are also highlighted. © 2005 Wiley‐Liss, Inc.