Topical and intermittent application of parathyroid hormone recovers alveolar bone loss in rat experimental periodontitis
Topical and intermittent application of parathyroid hormone recovers alveolar bone loss in rat experimental periodontitis
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DOI：
10.1111/j.1600-0765.2011.01386.x
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发表时间：
2011-12-01
影响因子：
3.5
通讯作者：
Nagata, T.
中科院分区：
文献类型：
--
作者：
Tokunaga, K.;Seto, H.;Nagata, T.
背景与目的：牙周炎是以牙周组织炎症和牙槽骨丢失为特征的一种牙周病。甲状旁腺激素（PTH），骨重建的主要调节剂，间歇给药已被证明刺激成骨细胞的活动。虽然全身使用甲状旁腺素可以预防牙周炎相关的骨质流失，但局部使用甲状旁腺素的效果尚不清楚。在这项研究中，间歇性管理的PTH对成骨细胞分化的影响进行了研究，在培养的颅骨细胞，然后局部和间歇性管理的PTH的影响，通过测量诱导实验性牙周炎后的牙槽骨丢失的恢复在rates.Material和方法：碱性磷酸酶活性和骨结节形成测定胎鼠颅骨细胞。用尼龙线结扎大鼠上颌磨牙20 d，造成实验性牙周炎。结扎线拔除后（第0天），局部注射PTH到颊牙龈，每周3次，共10周。结果：间歇性PTH暴露使大鼠颅骨细胞碱性磷酸酶活性和骨结节形成分别增加1.4倍和2.4倍。结扎程序诱导显着的牙槽骨周围的第0天的磨牙和更大的骨恢复观察PTH治疗的大鼠在第70天。在PTH治疗rats.Conclusion：间歇治疗PTH刺激成骨细胞分化胎鼠颅骨细胞培养，局部和间歇管理PTH恢复大鼠实验性牙周炎牙槽骨丢失的表面上的类骨质形成的增加。
Background and Objective: Periodontitis is characterized by periodontal tissue inflammation and alveolar bone loss. The intermittent administration of parathyroid hormone (PTH), a major regulator of bone remodeling, has been demonstrated to stimulate osteoblastic activity. Although the systemic administration of PTH has been reported to protect against periodontitis-associated bone loss, the effect of the topical administration of PTH is unclear. In this study, the effect of intermittent administration of PTH on osteoblastic differentiation was examined in cultured calvaria cells and then the effect of topical and intermittent administration of PTH was determined by measuring the recovery of alveolar bone loss after inducing experimental periodontitis in rats.Material and Methods: Alkaline phosphatase activity and bone nodule formation were measured in fetal rat calvaria cells. Experimental periodontitis was induced by placing nylon ligature around rat maxillary molars for 20 d. After ligature removal (day 0), PTH was topically injected into buccal gingiva three times a week for 10 wk. Micro-computed tomography analysis and histological examination were performed on days 35 and 70.Results: Intermittent exposure of PTH in calvaria cells increased alkaline phosphatase activity and bone nodule formation by 1.4- and 2.4-fold, respectively. Ligature procedures induced marked alveolar bone loss around the molars on day 0 and greater bone recovery was observed in the PTH-treated rats on day 70. An increase in osteoid formation on the surface of alveolar bone was detected in the PTH-treated rats.Conclusion: Intermittent treatment with PTH stimulated osteoblastic differentiation in fetal rat calvaria cell cultures, and topical and intermittent administration of PTH recovered alveolar bone loss in rat experimental periodontitis.