AN IMPROVED AUTOMATED PROCEDURE FOR DETERMINATION OF CALCIUM IN BIOLOGICAL SPECIMENS
AN IMPROVED AUTOMATED PROCEDURE FOR DETERMINATION OF CALCIUM IN BIOLOGICAL SPECIMENS
复制标题
DOI：
10.1016/0003-2697(67)90110-8
复制
发表时间：
1967-01-01
影响因子：
2.9
通讯作者：
GITELMAN, HJ
中科院分区：
文献类型：
--
作者：
GITELMAN, HJ
本文描述了一种改进的自动分光光度法测定钙的方法，该方法直接适用于血清、血浆、尿液和组织灰分样品。在8-羟基喹啉存在下，用CPC[甲酚酞络合物]比色法测定Ca，消除了显著的Mg误差。在分析从灰化组织制备的标本时可能遇到的铁干扰，已通过透析前用铜铁沉淀铁而消除。再现性研究，恢复数据，以及其他手动和自动Ca技术之间的比较。
An improved automated spectrophotometric method for Ca was described which is directly applicable to serum, plasma, urine, and tissue ash samples. Ca is determined colori-metrically with CPC [cresolphthalein complexone] in the presence of 8-hydroxyquinoline, which eliminates significant Mg error. Fe interference, which may be encountered in the analysis of specimens prepared from ashed tissue, has been eliminated by the precipitation of Fe with cupferron prior to dialysis. Reproducibility studies, recovery data, and comparisons between other manual and automated Ca techniques are presented.