The tail domain of PRRSV NSP2 plays a key role in aggrephagy by interacting with 14-3-3ε
The tail domain of PRRSV NSP2 plays a key role in aggrephagy by interacting with 14-3-3ε
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PRRSV NSP2 的尾部结构域通过与 14-3-3 epsilon 相互作用在聚合吞噬中发挥关键作用
DOI：
10.1186/s13567-020-00816-7
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发表时间：
2020-08-18
影响因子：
4.4
通讯作者：
Xiao, Yihong
中科院分区：
文献类型：
--
作者：
Cao, Shengliang;Liu, Jiaqi;Xiao, Yihong
由猪繁殖与呼吸综合征（PRRS）病毒引起的猪繁殖与呼吸综合征（PRRS）是影响几乎所有养猪国家的最严重的猪疾病之一。非结构蛋白2 （NSP2）是PRRSV生命周期中最重要的病毒蛋白之一。我们前期的研究表明，PRRSV NSP2可以诱导聚合体的形成。本研究探讨了聚集体形成对细胞的影响，发现NSP2可以诱导细胞自噬，细胞自噬依赖于聚集体的形成来激活聚集。NSP2的跨膜结构域和尾结构域参与了细胞的自噬，细胞蛋白14-3-3 epsilon通过结合NSP2的尾结构域在NSP2诱导的自噬中发挥了重要作用。这些发现提供了关于NSP2 c端结构域功能的信息，这将有助于揭示NSP2在PRRSV感染过程中的功能。
Porcine reproductive and respiratory syndrome (PRRS) caused by PRRS virus (PRRSV) is one of the most severe swine diseases that affects almost all swine-breeding countries. Nonstructural protein 2 (NSP2) is one of the most important viral proteins in the PRRSV life cycle. Our previous study showed that PRRSV NSP2 could induce the formation of aggresomes. In this study we explored the effects of aggresome formation on cells and found that NSP2 could induce autophagy, which depended on aggresome formation to activate aggrephagy. The transmembrane and tail domains of NSP2 contributed to aggrephagy and the cellular protein 14-3-3 epsilon played an important role in NSP2-induced autophagy by binding the tail domain of NSP2. These findings provide information on the function of the C-terminal domain of NSP2, which will help uncover the function of NSP2 during PRRSV infection.