Temperature and pyoverdine-mediated iron acquisition control surface motility of Pseudomonas putida
Temperature and pyoverdine-mediated iron acquisition control surface motility of Pseudomonas putida
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DOI：
10.1111/j.1462-2920.2007.01286.x
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发表时间：
2007-07-01
影响因子：
5.1
通讯作者：
Ramos-Gonzalez, Maria Isabel
中科院分区：
文献类型：
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作者：
Matilla, Miguel A.;Ramos, Juan L.;Ramos-Gonzalez, Maria Isabel
恶臭假单胞菌KT 2440不能在实验室常见的生长温度（30 ℃）下群集，但在18 ℃至28 ℃之间表现出与其他假单胞菌中的群集模式相似的表面运动性。这些运动细胞表现出分化，包括伸长和表面附属物的存在。对一系列突变体进行分析，以确定KT 2440中这种表面运动类型的分子决定因素，结果表明，IV型皮利和脂多糖O-抗原是鞭毛，而IV型则不是。虽然鞭毛突变体的表面运动是宏观上无法区分的野生型，显微镜分析显示，这些突变体移动使用一个不同的机制，野生型菌株。铁载体绿脓菌荧光素（ppsD）或FpvA铁载体受体中的突变体也不能在表面上扩散。ppsD菌株中的运动性用绿脓菌荧光素完全恢复，并且用野生型ppsD等位基因部分恢复。fpvA菌株的表型不被这种铁载体所补充。我们讨论了铁影响表面运动，它可以是一个环境线索的集群样运动在P. putida。这项研究构成了第一份报告，分配一个重要的作用，绿脓菌荧光素铁收购在energybacterial表面运动。
Pseudomonas putida KT2440 is unable to swarm at its common temperature of growth in the laboratory (30 degrees C) but exhibits surface motility similar to swarming patterns in other Pseudomonas between 18 degrees C and 28 degrees C. These motile cells show differentiation, consisting on elongation and the presence of surface appendages. Analysis of a collection of mutants to define the molecular determinants of this type of surface movement in KT2440 shows that while type IV pili and lipopolysaccharide O-antigen are requisites flagella are not. Although surface motility of flagellar mutants was macroscopically undistinguishable from that of the wild type, microscopy analysis revealed that these mutants move using a distinct mechanism to that of the wild-type strain. Mutants either in the siderophore pyoverdine (ppsD) or in the FpvA siderophore receptor were also unable to spread on surfaces. Motility in the ppsD strain was totally restored with pyoverdine and partially with the wild-type ppsD allele. Phenotype of the fpvA strain was not complemented by this siderophore. We discuss that iron influences surface motility and that it can be an environmental cue for swarming-like movement in P. putida. This study constitutes the first report assigning an important role to pyoverdine iron acquisition in en masse bacterial surface movement.