TRANSCRIPTIONAL REPRESSION BY THE HUMAN HOMEOBOX PROTEIN EVX1 IN TRANSFECTED MAMMALIAN-CELLS
TRANSCRIPTIONAL REPRESSION BY THE HUMAN HOMEOBOX PROTEIN EVX1 IN TRANSFECTED MAMMALIAN-CELLS
复制标题
DOI：
10.1074/jbc.270.46.27695
复制
发表时间：
1995-11-17
影响因子：
4.8
通讯作者：
CORTE, G
中科院分区：
文献类型：
--
作者：
BRIATA, P;VANDEWERKEN, B;CORTE, G
人类同源异型盒蛋白EVX 1（EVX 1）被认为在胚胎发生过程中发挥重要作用。在这项研究中，EVX 1对基因转录的影响进行了研究，在转染的哺乳动物细胞。EVX 1表达在多种哺乳动物细胞系中以浓度依赖性方式抑制由细胞特异性或病毒启动子/增强子序列指导的报告基因的转录。在我们测试的所有启动子中，转录抑制与EVX 1的DNA结合位点的存在无关。此外，EVX 1的阻遏作用在报道构建体中使用TATA较少的最小启动子时也是明显的。EVX 1的羧基末端脯氨酸/丙氨酸丰富的区域似乎是负责转录抑制活性，如EVX 1突变体的转染所示。我们推测EVX 1的阻遏物功能有助于其在胚胎发生中的作用。
The human homeobox protein EVX1 (EVX1) is thought to play an important role during embryogenesis. In this study, the effect of EVX1 on gene transcription has been investigated in transfected mammalian cells. EVX1 expression represses transcription of a reporter gene directed by either cell-specific or viral promoter/enhancer sequences in a variety of mammalian cell lines and in a concentration-dependent manner. Transcriptional repression is independent of the presence of DNA-binding sites for EVX1 in all the promoters we tested. Furthermore, repression by EVX1 is evident also using a TATA-less minimal promoter in the reporter construct. A carboxyl-terminal proline/alanine-rich region of EVX1 seems to be responsible for the transcriptional repression activity, as suggested by transfection of EVX1 mutants. We speculate that the repressor function of EVX1 contributes to its proposed role in embryogenesis.