Treatment relevant target immunophenotyping of 139 salivary gland carcinomas (SGCs)
Treatment relevant target immunophenotyping of 139 salivary gland carcinomas (SGCs)
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DOI：
10.1016/j.oraloncology.2009.05.635
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发表时间：
2009-11-01
期刊：
影响因子：
4.8
通讯作者：
Licitra, Lisa
中科院分区：
文献类型：
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作者：
Locati, Laura D.;Perrone, Federica;Licitra, Lisa
唾液腺癌（SGC）是一种罕见的肿瘤，涵盖广泛的组织学/生物学实体。标准的非手术治疗对于晚期疾病是无效的。我们的目的是分析 SGC 失调基因谱，这些基因谱可能成为创新治疗方案的目标。通过免疫组织化学方法对来自 139 名原发性、复发性和/或转移性 SGC 患者的样本进行了研究，以了解酪氨酸激酶受体 (TKR)（即 c-kit、HER2、EGFR）和激素受体（即雄激素 (AR)、雌激素 (ER) 和黄体酮受体）编码的蛋白质（PGR）。在 26 例病例中，荧光原位杂交分析补充了 HER2 免疫组织化学分析。EGFR 是表达最多的 TKR (71%)，在所有组织型中都有发现。c-Kit 表达主要限于腺样囊性癌 (78%)，而 HER2 表达主要通过基因扩增维持，在 44% 的病例中与唾液管癌 (SDC) 和未另有说明的腺癌相关 (AD, NOS）占 21% 的病例。就组织遗传学分类而言，除 HER2 外，TKR 表达更常见于闰管来源的肿瘤，而不是排泄性肿瘤。 AR 存在于 13% 的样本中，仅限于 SDC 和 AD、NOS，并且在超过一半的 SDC 病例中与 HER2 共表达。从未检测到 ER 和 PgR 阳性。这种 TK 激素受体分析确定了组织型特异性谱，可用于在前瞻性研究中更好地选择患者进行创新治疗。 (C) 2009 Elsevier Ltd. 保留所有权利。
Salivary gland carcinomas (SGCs) are rare tumors encompassing a wide spectrum of histologic/biologic entities. Standard non-surgical treatments are ineffective in case of advanced disease. Our aim was to analyze SGCs deregulation gene profiles that could become target for innovative treatment options.Samples from 139 patients with primary, recurrent and/or metastatic SGCs were investigated by immunohistochemistry for protein encoded by tyrosine kinases receptors (TKRs) i.e. c-kit, HER2, EGFR and hormonal receptors, i.e. androgen (AR), estrogen (ER) and progesterone receptors (PgR). In 26 cases, the HER2 immunohistochemical analysis was complemented by fluorescence in-situ hybridization analysis.EGFR was the most expressed TKRs (71%) and it was found across all histotypes.c-Kit expression was mainly restricted to adenoid cystic carcinoma (78%) while HER2 expression, mostly sustained by gene amplification, correlated with salivary duct carcinoma (SDC) in 44% of cases and adenocarcinoma, not otherwise specified (AD, NOS) in 21% of cases. With respect to histogenetic classification, TKRs expression occurred more often in tumors derived from intercalated duct rather than excretory ones with the only exception of HER2. AR was found in 13% of samples, restricted to SDC and AD, NOS and it was co-expressed with HER2 in more than half of the SDC cases. ER and PgR positivity was never detected.This TK-hormonal receptors analysis identify a histotype-specific profiles that could be exploited for better selecting patients for innovative treatment within prospective studies. (C) 2009 Elsevier Ltd. All rights reserved.