Hypoxia-induced oxidative stress and transcriptome changes in the mud crab (Scylla paramamosain)
Hypoxia-induced oxidative stress and transcriptome changes in the mud crab (Scylla paramamosain)
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DOI：
10.1016/j.cbpc.2021.109039
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发表时间：
2021-04-10
影响因子：
3.9
通讯作者：
Ye, Ling-Tong
中科院分区：
文献类型：
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作者：
Jie, Yu-Kun;Cheng, Chang-Hong;Ye, Ling-Tong
泥蟹（Scylla paramamosain）是中国重要的经济养殖品种。缺氧是青蟹养殖过程中的主要环境压力源。在本研究中，我们研究了溶解氧（DO）3.0 +/- 0.2 mg/L（称为“DO3”）的中度缺氧应激和DO 1.0 +/- 0.2 mg/L（称为“DO1”）的急性缺氧应激0、3、6、12和24小时后泥蟹鳃的氧化应激和转录组变化。 DO1的超氧化物歧化酶(SOD)活性在缺氧应激后3、6和24 h显着增加，而DO3的SOD活性在6和24 h显着增加。缺氧应激后6、12和24小时总抗氧化能力（T-AOC）显着增加。 DO1的丙二醛(MDA)浓度在缺氧胁迫后6、12和24 h显着增加，而DO3的MDA浓度仅在6 h显着增加。 DO1的乳酸脱氢酶(LDH)活性在缺氧应激后3、6、12和24 h显着增加，而DO3的LDH活性在12和24 h显着增加。对缺氧应激后24小时的鳃组织进行转录组分析。共获得1052个差异表达基因（DEG），其中DO1和DO3之间有394个DEG，DO1和对照组之间有481个DEG，DO3和对照组之间有177个DEG。 DEGs富集在与代谢、免疫功能、离子转运和信号转导相关的通路中。转录分析表明，糖酵解和三羧酸循环基因是调节青蟹适应缺氧胁迫的关键因素。
Mud crab (Scylla paramamosain) is an economically important cultured species in China. Hypoxia is a major environmental stressor during mud crab culture. In the present study, we investigated the oxidative stress and transcriptome changes in the gills of mud crab after intermediate hypoxia stress with dissolved oxygen (DO) 3.0 +/- 0.2 mg/L (named as "DO3") and acute hypoxia stress with DO 1.0 +/- 0.2 mg/L (named as "DO1") for 0, 3, 6, 12 and 24 h. The superoxide dismutase (SOD) activity of DO1 increased significantly at 3, 6 and 24 h after hypoxia stress, while SOD activity of DO3 increased significantly at 6 and 24 h. The total antioxidant capacity (T-AOC) increased significantly at 6, 12 and 24 h after hypoxia stress. The malondialdehyde (MDA) concentration of DO1 increased significantly at 6, 12 and 24 h after hypoxia stress, while MDA concentration of DO3 only increased significantly at 6 h. The lactate dehydrogenase (LDH) activity of DO1 increased significantly at 3, 6, 12 and 24 h after hypoxia stress, while LDH activity of DO3 increased significantly at 12 and 24 h. Transcriptomic analysis was conducted at 24 h of gill tissues after hypoxia stress. A total of 1052 differentially expressed genes (DEGs) were obtained, including 394 DEGs between DO1 and DO3, 481 DEGs between DO1 and control group, 177 DEGs between DO3 and control group. DEGs were enriched in the pathways related to metabolism, immune functions, ion transport, and signal transduction. Transcriptional analysis showed that glycolysis and tricarboxylic acid cycle genes were the key factors in regulating the adaptation of mud crab to hypoxia stress.