The carboxyl-terminal domain is essential for rhodopsin transport in rod photoreceptors
The carboxyl-terminal domain is essential for rhodopsin transport in rod photoreceptors
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DOI：
10.1016/s0042-6989(01)00195-x
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发表时间：
2002-02-01
期刊：
影响因子：
1.8
通讯作者：
Chen, J
中科院分区：
文献类型：
--
作者：
Concepcion, F;Mendez, A;Chen, J
利用转基因小鼠研究了羧基末端结构域在视紫红质转运中的作用，所述转基因小鼠表达缺少末端15个氨基酸的视紫红质截短突变体（S334 ter）。以前的研究表明，在内源性视紫红质存在下，S334 ter易位到外节。我们现在表明，在没有内源性视紫红质S334 ter错误定位到质膜，并未能重建外段结构。令人惊讶的是，这种错误定位并不影响感光细胞的存活。这些结果提供了进一步的证据上的重要作用的COOH-末端结构域的视紫红质运输，并证明了该域的绝对要求，正确的视紫红质在视杆光感受器的矢量运输。(C)2002爱思唯尔科技有限公司版权所有。
The role of the carboxyl-terminal domain in rhodopsin transport was investigated using transgenic mice expressing a rhodopsin truncation mutant lacking the terminal 15 amino acids (S334ter). It was previously shown that S334ter translocates to the outer segment in the presence of endogenous rhodopsin. We now show that in the absence of endogenous rhodopsin S334ter mis-localizes to the plasma membrane and fails to reconstitute outer segment structures. Surprisingly, this mis-localization does not affect photoreceptor cell survival. These results provide further evidence on the important role of the COOH-terminal domain in rhodopsin trafficking and demonstrate an absolute requirement of this domain for correct vectorial transport of rhodopsin in rod photoreceptors. (C) 2002 Elsevier Science Ltd. All rights reserved.