Diagnostic value of carcinoembryonic antigen combined with cytokines in serum of patients with colorectal cancer.
Diagnostic value of carcinoembryonic antigen combined with cytokines in serum of patients with colorectal cancer.
复制标题
癌胚抗原联合细胞因子对结直肠癌患者的诊断价值
DOI：
10.1097/md.0000000000030787
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发表时间：
2022-09-16
期刊：
影响因子：
1.6
通讯作者：
Wang, Xiaoqin
中科院分区：
文献类型：
--
作者：
Ma, Yunfeng;Zhang, Ya;Bi, Yu;He, Longmei;Li, Dandan;Wang, Dan;Wang, Mengying;Wang, Xiaoqin
在临床实践中，结肠直肠癌（CRC）很难与溃疡性结肠炎和结肠息肉区分开来。实用的标志物对诊断和治疗CRC患者是有用的。癌胚抗原（CEA）是诊断CRC患者的生物标志物。但CEA的诊断敏感性和特异性不高。白细胞介素（IL）-10、IL-17 A、肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）和转化生长因子-β（TGF-β）被认为与人类癌症的发生和发展密切相关。一些已被用作CRC的诊断标志物。目前尚不清楚细胞因子联合CEA是否可用作诊断CRC的生物标志物。采用酶联免疫吸附法检测大肠癌、溃疡性结肠炎、结肠息肉、胃癌和健康对照者血清中IL-10、IL-17、TNF-α、IFN-γ和TGF-β的水平。采用电化学发光法检测血清CEA水平。评估细胞因子联合CEA作为诊断CRC的生物标志物组的价值。CEA、IL-10、IL-17 A、TNF-α和TGF-β水平在CRC中显著升高。CEA的特异性高于其他细胞因子。IL-17 A、TNF-α和TGF-β在结直肠癌诊断中的敏感性高于CEA、IL-10和IFN-γ。血清CEA、IL-17 A和TNF-α联合检测对CRC的诊断效能更高（曲线下面积= 0.935）。 CEA、IL-17和TNF-α联合检测对结直肠癌的诊断效果优于CEA单独检测。含有IL-17 A、TNF-α和CEA的一组可能是一种有前途的分子生物标志物组，用于诊断性鉴别CRC与溃疡性结肠炎、结肠息肉和胃癌。
In clinical practice, colorectal cancer (CRC) is difficult to distinguish from ulcerative colitis and colon polyps. Practical markers are useful for diagnosing and treating patients with CRC. Carcinoembryonic antigen (CEA) is a biomarker for diagnosing patients with CRC. However, the diagnostic sensitivity and specificity of CEA are not high. Interleukin (IL)-10, IL-17A, tumor necrosis factor-alpha (TNF-α), interferon-gamma (IFN-γ), and transforming growth factor beta (TGF-β) are assumed to be closely related to the occurrence and development of human cancer. Some have been used as diagnostic markers in CRC. It remains unclear whether cytokines in combination with CEA could be used as biomarkers for the diagnosis of CRC. Serum levels of IL-10, IL-17, TNF-α, IFN-γ, and TGF-β in patients with CRC, ulcerative colitis, colonic polyps, stomach cancer, and healthy controls were measured by enzyme-linked immunosorbent assay. The serum level of CEA was detected using electrochemiluminescence. The value of the cytokines combined with CEA as a biomarker panel for the diagnosis of CRC was assessed. CEA, IL-10, IL-17A, TNF-α, and TGF-β levels were significantly increased in CRC. CEA displayed a higher specificity than the other cytokines. IL-17A, TNF-α, and TGF-β displayed higher sensitivities than CEA, IL-10, and IFN-γ in the diagnosis of CRC. The combination of serum CEA, IL-17A, and TNF-α achieved higher diagnostic efficacy for CRC (area under the curve = 0.935). The combination of CEA, IL-17, and TNF-α has better diagnostic efficacy than CEA alone in CRC. A panel containing IL-17A, TNF-α, and CEA could be a promising molecular biomarker panel to diagnostically differentiate CRC from ulcerative colitis, colon polyps, and stomach cancer.